That is very helpful. The test is the rendering of an interpretation or
diagnosis, not the mere operation of the machinery, correct?
Curt
-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
Sent: Tuesday, November 22, 2016 11:41 AM
To: Curt; Jesus Ellin
Cc: histon
I recently had this same discussion with Jesus, please follow this link he gave
me: http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfCLIA/Search.cfm
At the top, enter "Test System / Manufacturer" for example, we enter Ventana,
it will list all tests that qualify as High Complexity. There are
I would caution using Weigerts as a counterstain for IHC because it is
pretty dark in color and could be confused with the brown of the staining
you are looking for in your IHC. Have you tried Mayer's? That often
provides a pleasing stain with minimal effort.
You would counterstain after the DAB d
Could someone advise me on whether Weigert's iron hematoxylin can be used as
the counterstain for nuclei in IHC? We need to avoid using Harris's hematoxylin
because that will stain mucocytes in the coral tissues we are working with. If
it can be used, would that be after all antibody steps have
Curt, Well, that is the situation under the CLIA regs, but a deemed accrediting
agency, like CAP or JC, can implement stricter requirements. An institution can
do so as well (a medical center can require all its histology techs to qualify
for high complexity testing if it wants).
The ONLY issue
hematoxylin-eosin Y-Azure-II stain
Agree w Ren.
But try it anyway.
To bring out the azure,
you may wish to post fix.
Steve A. McClain, MD
> On Nov 22, 2016, at 13:29, "histonet-requ...@lists.utsouthwestern.edu"
> wrote:
>
> a hematoxylin-eosin Y-Azure-II stain
_
Curt,
Yes, but that is the test, not the personnel, and specifically applies to the
interpretation of the test, not the running of the machine. In AP the "testing
personnel" is the pathologist, not the tech running the machine. Only the
pathologist interprets the stains and reports a result. T
This should help...the IHC "test" is the pathologists' INTERPRETATION of IHC
stain. The stain procedure, validation of protocols and controls, and lot to
lot validation must be signed off by a pathologist.
Any tech that has demonstrated competency in performing the procedure, can
perform IHC s
I am going to attach the information where you can find what is high complexity
testing as defined by CLIA,, also CAP defines the QA/QC of this process to be
the high complexity ,, also the antibody workup,, here is the website where
you can get this information from. Again if you look at th
Jesus, that is very interesting information.
Does anyone know of any CAP accreditation documents that state explicitly that
IHC slide staining is high complexity? I have not seen any. If anyone has those
documents I'd like to see them. The only reference from CAP about that
classification I h
So I know I am going to open Pandoras box,, but have people been paying
attention to the Personal requirements from CAP.
I called the CAP and asked them about the criteria concerning Moderate or High
complexity testing, after discussing with them the situations, IF you have a
tech that is Lic
Tyrone:A.A.Maximow's Azur-eosin, etc staining produces wonderful results but
this, and many very old procedures, essentially rest on the use of mercury
salts which produce special chemical compounds with tissue components.Any, and
I mean any, deviation from the original procedure will not produc
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