Re: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium)

You can use either the sodium or potassium salt. Both can also be used to make Schiff's reagent as well. In fact, many histotechs leave out the sulphite rinse step and simply rinse off in water and wash well with tap water. It seems to work just as well. Bryan Llewellyn Angela Lamberth via H

[Histonet] AZ HT Position

HISTOTECHNICIAN Chandler Pathology Services - Chandler, AZ Job Type: Full-time Reports to: Laboratory Manager Schedule: 40 Hours per Week Salary: DOE FLSA Status: Hourly; Non-Exempt Essential functions and responsibilities: * Ensures compliance with all local, federal, CLIA, regulations.

[Histonet] Trimming soft brain

Hi there, I´m trying to trim ovine foetal brain (90 days of gestation). The brain is too soft and there is no way to get a proper slice. It has been in buffered 10% formalin for three weeks but still too soft. When you take the brain out of the pot and put into the trimming table, it just kind of

[Histonet] Shandon Cytoblock

Hi Folks, Does anyone have any experience/advice/feedback on the Shandon Cytoblock for cell block preparation? TIA - j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.we...@emoryhealthcare.org www.saintjosephs

[Histonet] Posting for a friend. Unlicensed PA's and what they can qualify to gross

Hi Histonetters!! I am posting these questions for a friend: What can a non licensed person with 12 or more science credits gross in a surgical pathology lab? What can a licensed Histotech with a 2 year degree, with the training from a Pathologist qualify to gross in a surgical Pathology lab? What

Re: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium)

Thank you Philip. I'm writing a procedure and was driving myself bananas. On Mon, Mar 6, 2017 at 11:01 AM, Manfre, Philip wrote: > I almost always have used SODIUM metabisulfite for any version of PAS. I > am also familiar with procedures that go straight to tap water for the > pot-Schiff's rin

Re: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium)

I almost always have used SODIUM metabisulfite for any version of PAS. I am also familiar with procedures that go straight to tap water for the pot-Schiff's rinsing, though that was from a job in my past. I seem to remember that working fine. Phil. Philip Manfre, HT (ASCP) Associate Principa

[Histonet] UV bulb in cryostat

I am trying to justify the $1600 cost of replacing the uv bulb in one of my cryostats. The 2 other cryostats I have do not even have a uv bulb in them, but I have one tech who is insisting that I replace the bulb. I have been told by management that it's too expensive and since the other instrument

Re: [Histonet] TUNEL

Hi Bernice, Likely there is something in your TUNEL procedure that is causing a problem with histochemical nuclear staining. If it isn't the pre-treatment, it might be the DAPI. After all, they do bind the same structures, and the DAPI might be winning that competition. Teri Johnson Manager, C

[Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium)

In Carson’s 3rd edition, the reducing rinse following Schiff for PAS is 0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing rinse is given as sodium metabisulfite (Carson pg 149). My other texts (Sheehan/Hrapchak as well as Vacca) list the reducing rinse as sodium metabisulfite w

Re: [Histonet] Newbe help

Start with this link and navigate through the website. There are various online and in-person programs. Best of luck. http://nsh.org/content/schools -Original Message- From: Keith Brown via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, March 06, 2017 11:09 AM To: hist

[Histonet] Newbe help

Hi. I've looked through the archives of this group to little avail. If i missed an answer to my questions that was previously posted to this list, my apologies. I'm looking to get into the histotech field, but I'm not sure how to go about it. I'm changing careers after 20 years as a journalist. I

[Histonet] Histotechnology Professionals Day is March 10, 2017.

Dear Histonetters! This Friday March 10 is Histotechnology Professionals Day!! The National Society for Histotechnology has some great suggestions for ways to celebrate. Here is the link: http://www.nsh.org/content/histotechnology-professionals-day Happy Histotechnology Professional

[Histonet] GSH Annual Histopalooza April 21 - 23 Legacy Lodge Lake Lanier Islands, Buford, Georgia

The annual Histopalooza is just around the corner. The annual event is April 21 - 23 . Please visit our website for details: http://www.histosearch.com/gsh/ Reserve your room by March 21, which is only 15 days away. Registration for the event is $135 prior to April 10th. What a steal compared

[Histonet] CPT code 88342

Good Morning Histonetters, For Meditech users who download 3M files for medical necessity, has anyone had any issues with CPT code 88342 rejecting and only accepting diagnosis codes specific to lung (dx code C34... ). Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tide