Re: [Histonet] Tissue Fixation

2017-04-02 Thread Tony Henwood (SCHN) via Histonet
If the specimen is placed on absorbent paper prior to fixation, the paper will gradually absorb the water from the specimen causing it to dry out. Microscopically the tissue will look a little like what you see when a laser scalpel is used to excise a skin specimen. The heat affected eosin stain

Re: [Histonet] Histonet Digest, Vol 161, Issue 2, "SURVEY"

2017-04-02 Thread Brent Adams via Histonet
In our GI Laboratory we have both and we do between 35 and 55 blocks per day. I would not want to have to choose between the both. I would get a "new" Auto stainer because the quality of your stained slides would be more consistent and be easier to assess for QC purposes. I would get a "use

Re: [Histonet] Survey!!!!!!

2017-04-02 Thread Frazier, John via Histonet
The one thing I would do is to make sure you choose a glass coverslip. Plastic is not good over time and varying temperatures. Sent from my iPad > On Apr 1, 2017, at 7:18 PM, Patsy Ruegg wrote: > > I have seen so many more problems with auto coverslippers, they break down, > the cover does not

Re: [Histonet] Survey!!!!!!

2017-04-02 Thread Rene J Buesa via Histonet
Staining is a more complex step than coverslipping. Also a coverslip on a section has more "latitude" and can, if necessary, be removed and coverslip in a different way. A stained section, on the other hand, is almost impossible to "correct". Between an autostainer and a coverslipper, I would ch