[Histonet] H&E on frozen sections

2017-05-02 Thread Ana Maluenda via Histonet
Hi everyone, Just wondering if anyone out there had already had issues with commercial Haematoxylin and Eosin. I started working in a lab that recently purchased Sigma Mayer's Haematoxylin (MHS-16) and 1% Alcoholic Eosin (Fronine - II016). We work with fresh frozen sections of mouse tissue. Sin

[Histonet] Gold Chloride Source

2017-05-02 Thread ian bernard via Histonet
Histonetters, looking for Gold Chloride purchase source. Current distributor has the product on back order- Sigma Aldrich. Need another source since we are running low. Please provide catalog number as well. IB ___ Histonet mailing list Histonet@

[Histonet] Cryostat and Parts

2017-05-02 Thread Travis Troyer via Histonet
We are in the process of moving and we have some equipment and supplies that we are not taking with us.We have 2 Microm HM 505E cryostats and all of the internal components.One of the cryostats doesn't cool down and the other one doesn't advance properly but is otherwise in working order.

Re: [Histonet] . Refurbished Leica RM2135

2017-05-02 Thread Mayer,Toysha N via Histonet
Sandra, On some holders you can easily convert back and forth from low to high. If your service person does not know anything about that, I would ask Leica. Specifically ask for a tech rep with the most microtome experience (in years). That person should be able to tell you what part you nee

Re: [Histonet] Refurbished Leica RM2135

2017-05-02 Thread Пешков Максим via Histonet
From sugh expensive blades can be done more very good sections (at 3 microns) from any difficult type tissue. These investments will pay off very quickly. Maxim Peshkov Russia, Taganrog. >Вторник, 2 мая 2017, 21:16 +03:00 от "Howery, Jeff L" >: > >But, high profile knives are more expensive. T

Re: [Histonet] Refurbished Leica RM2135

2017-05-02 Thread Пешков Максим via Histonet
Sandra, high profile blades was better for me, because they are harder and more durable than low profile of any manufacturer. Also I recommend for you to try Surgipath DB80HS. They must better than 818. I regret that not having bought a holder for a high profile blades. Leica makes the blade ho

[Histonet] AFB Control Blocks

2017-05-02 Thread Clarence Owens via Histonet
Good Morning All, I am in search for 2 blocks of AFB positive lung blocks. Does anyone have 2 blocks they could spare. I am willing to trade a control block for another control block. I thank you for your help in advance. Regards, - Clarence Owens ​, HT (ASCP) QIHC​ _

[Histonet] Refurbished Leica RM2135

2017-05-02 Thread Sandra Cheasty via Histonet
Hi everyone, I have a newly refurbished Leica RM2135 at my disposal, but the knife holder is for high profile blades. 2 questions. 1-Is it that different using high-profile blades vs. low. (I have used low-profile blades forever.) 2-There is a slot on the bottom of the blade holde