[Histonet] PIN4

2017-08-04 Thread Renee Fisher via Histonet
Need help the PIN4 has suddenly stop working we use P63+CK5+CK14 from Bio Care Medical and P504S from Dako. We double stain on the Leica Bond with the Polymer refine Red and Polymer Refine detection. This stain has worked beautifully in the past. Any suggestions? Thank you _

[Histonet] new dako omnis machine vs current dako autostainer link 48

2017-08-04 Thread cafechague via Histonet
hi all!I'm validating our dako omnis. it is a brand new platform.  and I'm aware that ten positive and negative controls for antibody validations are needed for cap regulations.we are currently using the older dako stainerlink48 with all our antibodies already validated, but plan on moving it to

[Histonet] biobond experience?

2017-08-04 Thread Travers, Susan via Histonet
Hi all, Just wondering if anyone has had any experience using biobond as an adhesive for coating slides. We have used a chrome-alum/gelatin coating for years with formalin-fixed, frozen brain sections and it works pretty well. However, with certain stains (e.g., black-gold) we continue to have

Re: [Histonet] Adenovirus antibody?

2017-08-04 Thread Jan Shivers via Histonet
Hello Paula, This isn't an answer to your question, because I do use the Millipore antibody (MAB805) and it works quite well on a Dako platform or done manually. It requires enzyme digestion antigen retrieval. I use it at a 1:400 dilution (45 min) with a polymer detection system (45 min). I'm n

[Histonet] Happy Friday--Lab related blog post

2017-08-04 Thread Lester Raff MD via Histonet
Have a good weekend-and I hope your lab works like ours! http://www.chicagonow.com/downsize-maybe/2017/08/we-are-great-because-you-wont-stop/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203