We are a small derm lab and have a Nemesis 3600 from Biocare. It will
perform IHC on up to 36 slides per run and is an open platform. We also do
a reagent rental through them. The doctor is happy with the quality.
On Fri, Mar 16, 2018 at 2:25 PM, Eddie Martin via Histonet <
histonet@lists.utsou
Dr Fleming,
If you’re insistent on automation, I would suggest a used Leica Bond Max
instead. The Leica Bond Max is most likely the most cost effective, and has
the smallest footprint while also minimizing reagent waste compared to a Dako
Flex or Ventana XT/Ultra.
If you are willing to do it
For the validation, yes you have to run negative cases. This is to verify that
tissue you know should not stain, does not do so. For example, let's say you
are validation H.pylori. You have 10 cases where you know the patient has
H.pylori bacteria in a gastric biopsy (positive staining). Yo
Keep in mind like Lacy said its cases and not slides so you could place
multiple cases on one slide or create a simple array using disposable biopsy
punches.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060
Hi Paula,
Let me first say I am Canadian and my lab is not governed by CLIA or CAP.
But for what it is worth, here are my thoughts...
When thinking about validating antibodies for IHC you must first consider
whether the antibody in question is Class-I (prognostic eg Breast markers,
CD117, etc) or
CAP suggests running 10 negative and 10 positive cases for non-prognostic
markers.
CAP required running 20 negative and 20 positive cases for prognostic markers.
In the event the case volume is less than the suggested 10 and 10 cases for
non-prognostic markers, the reason for that decision sho
Hello,
We've been discussing about the quantity of slides to run as a validation
for IHC antibodies. We are governed by CLIA, and we would like to know if
there is a set number of slides to run for a particular antibody we would
like to bring in-house for Validation. I think CAP requires 20 sl
Hi,
I have a small dermatopathology lab and would like to introduce limited
immunohistochemistry. Initially I would probably just do MITF stains with
red chromogen on a few cases per week. We could do this by hand but I've
noticed a number of ST5050 immunostainers for sale at very low prices. I
k
