Hi,
> I have a hunch that the DAB deposits you are seeing are not a DAB
problem. You didn't mention how long your incubation time is. If I were to
venture a guess, I am thinking there may be some evaporation of either the
primary or secondary antibody or boil-over from the antigen retrieval.
I was taught it best to use separate controls on a second slide. But in
practice, the only cases where a floater appeared it was obvious. In my own lab
14 year experience it has not happened.
However, about 10 years ago we started an experiment and switched to 2 AFB
slides with pos and neg cont
Can anyone using a Dako Artisan give me an idea (ballpark is fine) of what your
cost per slide is running? Thank you in advance!
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Good morning,
I am currently experiencing an issue with DAB concentrating on certain bone
areas of my slide. I am working with decalcified—EDTA , FFPE- rabbit mandible
(some tissue attached). It is a new DAB kit from BioCare. It’s thoroughly mixed
(and new) before application in a humidity cham
