Please see my protocol, we successfully use it on murine liver and hearts.I do
not stain nuclei, in my opinion counterstaining with any type of hematoxylin
contaminates and fades picris acid.
- Sirius Red F3BA(Polysciences #09400), (Direct Red 80 from Sigma Chem.
Co. (Cat# D 030))
-
I suspect the "all red" as opposed to "only some red" is because the PSRed
is not de-staining out of the non-collagen structures. My guess is that
your Solution B is old. Acetic acid becomes weak in water very fast so I
make the 1% acetic acid solution fresh every time and use the same day.
Hope
The sirus-red stain is a stain to differentiate collagene I against collagene
III in polarized llight, so in bright field all fibres will be red, just if you
change to polarised light, you can differentiate both kinds of fibres by their
birefrigence which is orange to yellow for collagene 1 and
