There are several papers on this. We did a purposeful underprocess and cut and
stained it, then reprocessed as normal and compared. The results were
eye-opening in terms of how good it worked, but you need to factor in that the
second processing will diminish IHC staining quality.
Get Outlook f
Thank to those who responded so quickly. It happens maybe once a quarter. It
happened today because the programs got switched: large tissue processed on
biopsy program. I've heard of folks doing it this way with the theroy the
paraffin protects the processed tissue. I'm just old school I guess
Hi Cassie
How often is this happening? We have done it this way, with no ill effects on
the processor, but it comes up maybe once every few months - if you have to
reprocess tissue daily or weekly, I could see it adding up to a problem. We
melt the block, blot the tissue and cassette with gauze
Has anyone had success with just melting down unfaced, underprocessed blocks
for reprocessing and sticking them in NBF for processing without taking them
through the clean cycle? The senior tech is doing this and I am concerned about
our processor getting paraffin in the NBF and other things.
C
Hello Histo colleagues!
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