Kristy:
I’m thinking along the same lines as Paula; is it possible that your detection
reagent contains only Alkaline Phosphatase (ALP) as there a reactive enzyme?
If so, a peroxide/DAB solution simply won’t react with it. Can’t wait to see
your protocol, with detailed descriptions of the ant
Since you are newbies, I am just putting IHC 101 out there.
Permanent red is for alkaline phosphatase detection and DAB is for HRP.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com
A sharp
Thanks for the tip!
On Fri, May 1, 2020 at 8:53 AM Val Tolley wrote:
> It’s difficult to know without your full protocol, but you can easily test
> your DAB.
>
> Take a paper towel and add a drop of your secondary/HRP. Add a drop of
> DAB to that. If it doesn’t immediately turn brown, your hyd
It’s difficult to know without your full protocol, but you can easily test your
DAB.
Take a paper towel and add a drop of your secondary/HRP. Add a drop of DAB to
that. If it doesn’t immediately turn brown, your hydrogen peroxide has likely
degraded into water. Make a fresh batch of DAB and
Hydrogen Peroxide gone bad / or not being added???
From: Kristy Castillo via Histonet
Sent: Friday, May 1, 2020 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC DAB
Hi Histonetters,
We are starting our IHC (fun times), we are having troubl
Hi Kristy,
What platform and detection kit are you using? Would you mind sharing your DAB
protocol?
Thank you,
Daniel Pesino, HTL(ASCP)CM QIHC CM
Senior Histotechnologist
Trinity Health Of New England
daniel.pes...@trinityhealthofne.org
W 860-714-4675
114 Woodland Street
Hartford, CT 06105
T
Hi Histonetters,
We are starting our IHC (fun times), we are having trouble with the DAB
lighting up. Processed for 5 thru 10 minutes and still nothing. Our
permanent red is working just fine. Any ideas.
Thank you!
Kristy
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Hi all,
We have a Leica ST5010 that we have had forever and love. We have gotten a lot
busier and need to eliminate the backup we currently get every day waiting to
load the racks. I think we need an extra oven or 2. So we are looking into
updating and will probably go with the ST5020 or the
