Make sure sodium azide (antimicrobial preservative) is not used in any of your
IHC reagents--it inhibits peroxidase.
Mike
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Message: 3
Date: Fri, 1 May 2020 08:00:49 -0700
From: Kristy Castillo <k.casti...@azdermgroup.com>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC DAB
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<CADFdJFAPWF_puvNe+ATKDwWoguN4GueLYq=noexa-uddon+...@mail.gmail.com>
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Hi Histonetters,
We are starting our IHC (fun times), we are having trouble with the DAB
lighting up. Processed for 5 thru 10 minutes and still nothing. Our
permanent red is working just fine. Any ideas.
Thank you!
Kristy
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