[Histonet] Prussian Blue Reaction

2021-06-05 Thread Mac Donald, Jennifer via Histonet
Does anyone know of a way to remove/reduce the Prussian blue reaction? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Protocol for DAPI staining on paraffin sections

2021-06-05 Thread John Kiernan via Histonet
Not everyone knows that the product of the Feulgen reaction is fluorescent. If you do the method on paraffin sections, DNA in nuclear chromatin is red with ordinary illumination. If you look with a fluorescence microscope (blue excitation) it shows as a brownish-red fluorescence in the chromatin