So I am working with Canine femurs and tibias that have been dissected as medial and lateral - they have been decaled in formic acid - rinsed in running tap well - processed on a routine 1 hour processing program ..... cutting very well!!
My issue is that the articular cartilage on the bone surface keep flipping up and over onto itself - I am using a 37C slide warmer overnight to allow them to hopefully flatten out more than on the water bath and then drying in the 56C oven for about 1 hour - then staining on automated Leica stainer - they are intact at 37..... look like the might be curling at 56C?? Anyone have any suggestions??? TIA - Amy Amy S. Porter, HT (ASCP) - Lab Supervisor Michigan State University Investigative HistoPathology Lab 567 Wilson Road - Rm 2133 East Lansing, MI 48824 Ph: 517-884-5026 (she/her/hers) https://sites.google.com/msu.edu/ihpl/home<https://urldefense.com/v3/__https:/sites.google.com/msu.edu/ihpl/home__;!!HXCxUKc!gaNWP-BGXxBxNF3HkuINL65j6K4xY5fN6q1y0Pb9w0qNLYZjcV7-CHy_BQT42A$> "Life is like riding a bicycle. To keep your balance, you must keep moving." - Albert Einstein _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
