I don't know anything about "Jore's fixative" or the rationale of using a very
hypertonic unbuffered 4% formaldehyde with magnesium, sodium, chloride and
sulphate ions. If brown stuff is now bleeding out of your museum specimens,
Jore Juice evidently isn't a good preservative.
According to Chap
Thought on alternative for Sudan Black. I don't use this stain...an
alternative is an Oil Red O stain. Oil Red O is done on frozen
sections...but you can also deparaffinize FFPE sections to water, and then
perform your Oil Red O stain.
I hope this helps.
Very Respectfully,
Eddie Martin
Eddie M
Dear histoneters,
I have performed heating antigen retrieval with citrate buffer
pH 6 with 0.05% tween-20, however I have seem recipes with no detergent,
anyone has any experience or knowledge if it is better with or without the
detergent?
Thank you!
--
Dr. Alonso MartÃnez C
Hi All,
I am looking to replace the fixative for veterinary specimens that have been
preserved as "museum specimens". They are kept in jars in a glass case outside
our lab, however, some of the fixative is starting to turn brown (and we've
pulled a few jars that have some slight cracks in them).