Re: [Histonet] Coverslipping mystery

Does this sound plausible? No problem with the pertex and pipettes (which is what I've used for years with no issue) Thanks Adam -Original Message- From: Caroline Miller [mailto:mi...@3scan.com] Sent: 11 July 2015 15:18 To: John Kiernan Cc: Adam Boanas; histonet@lists.utsouthwestern.

[Histonet] Coverslipping mystery

occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Frozen section artefact

Hi again, In your opinion is it better to fix frozen sections prior to storage in -80 or following removal from -80 prior to a run? Thanks Adam -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl

RE: [Histonet] Frozen section artefact

Thanks for the reply - Previously I was going directly from the fixative to the PBS. Yesterday I tried air drying the sections for 30mins prior to PBS and the sections looked much better. Will try increasing the alcohol content as you suggest. Adam -Original Message- From: histonet-boun

[Histonet] Frozen section artefact

s, Gut Mucus (have stained PAS to highlight), nuclear degredation. Could it be fixation? We air dry following sectioning and fix in Acetone / alcohol 3:1 for 5 mins. Any ideas would be great! Thanks in advance. Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9

[Histonet] Translucent patch

tissue causing this patch? Any ideas would be great, Many thanks, Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

[Histonet] Whiskers

soften the keratin prior to embedding? I am thinking 10% Sodium Hydroxide or 5% Ammonium Hydroxide but do not really know about appropriate timings for such a small tissue. Many thanks, Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX

[Histonet] Elastin Aldehyde Fuchsin

e any idea about what this is and how it could be prevented? (Our next guess would be to re-filter the fuchsin solution prior to use every time) Many thanks Adam Adam Boanas Histology Manager Renovo Ltd PLEASE NOTE OUR TELEPHONE NUMBERS ARE CHANGING -PLEASE AMEND YOUR RECORDS: >From Mond