[Histonet] Embed-It

Hello all, We are getting ready for a project that uses Embed-It(tm). We are embedding decalcified bones in the plastic to obtain 0.5um sections. Has anyone had any experience with this product? It looks like a fairly simple kit. Much different than the Ostoebed(tm). Any helpful hits would be gr

RE: [Histonet] Frozen brains on cryostat

Hi Andrea, I'm not sure about the unfixed tissue, but we got awesome advice from Donna Emge in regards to our wrinkle problems on fixed frozen brains. We would dip a brush in water and run that across the slide (not too much and not too little), then pick up the tissue. My first thought was, "Wa

[Histonet] 2% low melting agarose gel

Hello, Has anyone had any experience with cutting cell mixed with 2% low melting agarose gel? The cells were fixed in 10% NBF, mixed with the gel and then processed to a paraffin block. I've tried a couple of techniques, but the end result is mush. Any ideas or suggestions? Bobbie Boyce Histol

RE: [Histonet] Toluidine blue

I put it in a translator program and it's something like this: long time ago: you know anything about the application of cd4 on varkensweefsel formalin fixed and in paraffin embedded fabric? have another question: are you in the possession of a cryostat where you still with old-fashioned fixed kni

[Histonet] Frozen eye problems

Hello Histoneters, I'm having problems getting a good section on paraformaldyehyde fixed adult mouse eyes frozen in TBS tissue freezing medium. One of the problems was the orentation of them. They were too close to the edge. I also, wanted to try a harder freezing medium, so I corrected oriented a