Hi Bryan,
Good to hear from you! How are you keeping?
You are quite correct regarding Baker's data.
He engages in some discussion on this matter, mentioning the microscopic
shrinkage and gives data on both whole cell volumes as well as nuclear
volumes.
I don't find his figures high but actuall
Richard,
It depends upon the tissue type, the fixative used, time/temperature/
gradation of alcohols, intermediate solvent type/time/temp and the wax
time/temp.
Careful fixation and processing of liver or spleen, using 4% aqueous
formaldehyde/ graded alcohols /xylene/ wax at 56-60C, results in
Adele,
Since the vanGieson IS a trichrome stain, why would anyone wish to combine
one trichrome with another?
Bryan
- Original Message -
From: "Doughty, Adele"
To:
Sent: Monday, July 19, 2010 10:29 AM
Subject: [Histonet] vanGieson-Trichrome staining protocol
Is there anyone ou
Mike,
There are a number of yellow counterstains commonly used, including picric
acid.
For your purpose, I would recommend the following in order of preference;
Tartrazine (Acid yellow 23, Food yellow 4) CI 19140,
Metanil yellow (Acid yellow 31) CI 13065,
Martius yellow (Acid yellow 24) CI 103
hours spent hand sharpening
the Heiffor knives!!
I still have two original Wilkinson sword knives in my possession, although
they are a little worse of wear now.
Once again, thanks for the memories.
Bryan Hewlett
(retired histotechnologist)
- Original Message -
From: "Gordon
Kris,
It is a general oversight stain as is the H&E.
It is correctly refered to as the Masson Hematoxylin-Phloxine-Saffron (HPS)
stain.
Although some people substitute eosin for phloxine, this is a generally
inferior technique to HPS (I have NEVER seen a good one!).
Technically, the method is h
Kristen,
It's more likely due to the presence of lipids extracted from the tissues.
How often do you replenish/change alcohols?
Bryan
- Original Message -
From: "kristen arvidson"
To: "histonet"
Sent: Monday, March 15, 2010 1:06 PM
Subject: [Histonet] Contamination on Processor
We
Perform the Alcian blue first, then follow with the H&E.
Done it hundreds of times, no problem.
Bryan
- Original Message -
From: "Perry, Margaret"
To:
Sent: Monday, December 14, 2009 11:17 AM
Subject: [Histonet] alcian blue stain
We are really getting new research procedures lately
Hi Gayle,
You are generally correct.
Essentially, the only proteins denatured (fixed) by acetic acid are the
nucleoproteins.
However, 5% aqueous acetic acid has been used as a fixative for chromosomes
in fresh material (commonly plant).
We used this in school to see mitosis in onion root tip,
Hi Tim,
You gotta luv this! Where do they find these auditors?
I can understand the requirement for the use of negative stain controls for
definable entities,
but for general oversight and other differential general tissue element
stains, I am at a loss!
What would be required is a tissue wi
Daniel,
You wrote;
That said, is there a reason why we couldn't or shouldn't
process breast core Bx's on the Xpress provided they've been sitting
overnight in formalin?
See following article.
Concensus Recomendations on ER testing in breast cancer by IHC(Appl
Immunohistochem Mol Morphol 2008
Hi Ian,
For paraffin wax infiltration, the normally quoted range of vacuum pressures
to use varies between 360 mmHg(48 kPa) and 260 mmHg(34.66 kPa).
I always reduced the pressure slowly in the first wax to just 360 mmHg to
prevent violent 'bumping' due to rapid outgassing of the clearant.
Seco
Richard,
My quick and easy test for muscle striations is to check the H&E under
crossed polarizers.
Myosin is birefringent. The birefringence can be intensified by staining
with picric acid, e.g. following a Van Gieson stain.
Regards,
Bryan
- Original Message -
From: "Edwards, R.E.
Hi Bill,
Phenol-formalin fixative consists of the addition of 2% w/v phenol to 4%
formaldehyde in phosphate buffer.
It has been recommended that sequential fixation in phenol-formalin at pH7.0
followed by phenol-formalin at pH5.5, provides superior results.
I am attaching the reference in a se
Imelda,
Chlorantine fast red 5B, AKA direct red 81 and Sirius red 4B, CI 28160 is
listed by VWR International UK(tel 800-932-5000).
Bryan
- Original Message -
From: "McGonnell, Imelda Mary"
To:
Sent: Monday, April 20, 2009 9:38 AM
Subject: [Histonet] suppliers of chlorantine fast
Tom,
The alkaline Congo red method for amyloid will also stain cellulose.
Examined by polarisation microscopy, only amyloid and cellulose will give
the characteristic green dichroic birefringence.
In addition, Congo red is fluorescent.
Bryan
- Original Message -
From: <[EMAIL PROTECT
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