[Histonet] New Lab

2019-01-11 Thread Campbell, Tasha M. via Histonet
Hello, I am a small GI lab and our facility is moving to a brand new building! The building has yet to be built so I have full control on how to design my lab. I will have about 1000-1500 sq ft. I was just wondering if anyone had any tips for me? One thing I want to change is the type of flo

[Histonet] Artifact

2018-11-16 Thread Campbell, Tasha M. via Histonet
Can anyone help me out with the artifact I am getting on my slides? It is on all of them, IHC, H&E, specials. It looks like formalin pigment, but I use 10%NBF so should I really be getting formalin pigment this much on my slides? I am a GI lab. I use microwave processing. Specimens are held 2

Re: [Histonet] test

2018-11-16 Thread Campbell, Tasha M. via Histonet
-Original Message- From: Eileen Akemi Allison [mailto:akemiat3...@gmail.com] Sent: Friday, November 16, 2018 11:01 AM To: Campbell, Tasha M.; Histonet Subject: Re: [Histonet] test ** CAUTION: This email originated from outside of Frederick Memorial Hospital. DO NOT click on links or

[Histonet] test

2018-11-16 Thread Campbell, Tasha M. via Histonet
I keep trying to send an email for help with an issue I am having and its not going through. It says the email has been sent but I am not getting the email and no one has responded. I am testing to see if this message goes through. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology As

[Histonet] Control Blocks

2018-10-04 Thread Campbell, Tasha M. via Histonet
Does anyone happen to have or know where to get HSV I and II and CMV control blocks? The control slides are so expensive and it would be so much easier to have a block that will last a long time and give me many for slides. Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology

[Histonet] DI Water

2018-10-02 Thread Campbell, Tasha M. via Histonet
I am looking to install a small DI system or reverse osmosis system in my small GI lab. We are bringing IHC in house and I thought it would be cheaper and easier in the long run to have a DI system. I am not sure what type to get though. There are so many out there. I don't want anything too

[Histonet] Iron Stain

2018-08-08 Thread Campbell, Tasha M. via Histonet
Is nuclear fast red the only counter stain for Prussian blue stain? I have a Masson's trichrome kit and was wondering if the Bierbech Scarlet could be a counterstain. I won't be doing the iron stain very often at all so I am trying to keep dry reagents on hand to make up as needed so they do n

Re: [Histonet] Paperwork

2018-07-27 Thread Campbell, Tasha M. via Histonet
: WILLIAM DESALVO [mailto:wdesalvo@outlook.com] Sent: Friday, July 27, 2018 1:20 PM To: Campbell, Tasha M.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paperwork I have a few suggestions: Batch control - you do need to continue documentation of which cases/slides corresponds to

[Histonet] Paperwork

2018-07-27 Thread Campbell, Tasha M. via Histonet
Hi everyone, I have 2 questions: 1. Could someone please share some ways to keep track of the control that goes with the slides that it was used for? So I am a small GI lab and there is a pathologist here a couple days a week. We do trichrome on Microscopic colitis cases and so I have be

[Histonet] Masson's Trichrome Troubleshooting

2018-05-30 Thread Campbell, Tasha M. via Histonet
Hello all, I am having issues with my trichrome stain and I am about to lose my mind! We just started doing it in house (although at my previous job I had done trichrome by hand for years so I am not a stranger to it. And I never had issues with it). When I brought it in house at my current l

[Histonet] IHC Platforms

2018-04-16 Thread Campbell, Tasha M. via Histonet
Hello everyone. I was wondering if any small, GI labs do IHC's in house and if so what platforms you use. I am just researching right now to see if it would be worth it to do in house or not. I know a lot of these instruments and reagents are expensive so I would be looking for the most inexp

[Histonet] Used equipment

2018-01-25 Thread Campbell, Tasha M. via Histonet
Anyone know who to contact to sell used equipment to? Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu h

[Histonet] Milestone RHS-1 Accessories

2017-05-17 Thread Campbell, Tasha M. via Histonet
Hi all, Does anyone have any of the histomodules for the milestone RHS-1 110 cassette rack they would like to sell? I need 3. I need the glass containers, lids, stirrers, carriers, the disks and the heating disk. Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associate

[Histonet] Shurwave Processor

2017-04-12 Thread Campbell, Tasha M. via Histonet
Hello all, Is there anyone that can help me with my Shurwave Microwave Processor? The unit is very slow reaching temp. I have to double the times of all steps for it to get up to temperature. I have replaced both magnetrons, filament transformers, high voltage transformers, capacitors, solid

[Histonet] PRN Frederick, MD

2017-01-16 Thread Campbell, Tasha M. via Histonet
Hello all, I am looking for a histotechnologist in the Frederick, MD area that would be interested in covering vacations and sick time for a small GI lab. Flexible hours. Easy job. Please email or call if interested. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310

[Histonet] Clia Competency Form

2016-10-06 Thread Campbell, Tasha M. via Histonet
Does anyone have a CLIA competency form for histology supervisor, histotechs, and technical supervisors that they would be willing to share? It would be greatly appreciated!!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800

[Histonet] water on slides

2015-09-03 Thread Campbell, Tasha M. via Histonet
I've been having this problem for a month or so. It is very humid in my lab right now because of the weather here so I am very cautious about changing and rotating everything everyday. But I am having issues with water being in the first slide of the first run everyday! Its so weird! That i

[Histonet] Temporary Histotech Needed

2015-09-03 Thread Campbell, Tasha M. via Histonet
Hello everyone, I was wondering if there is anyone out there that does temporary work in histo labs in the Frederick MD area. I run a small GI lab and I will be going on maternity leave in October and we would like someone to fill in. I know that there are temp agencies I can go through, but I

Re: [Histonet] Billing question

2015-05-08 Thread Campbell, Tasha M.
>From my understanding its just one charge. Its per specimen such as A, B, C, >etc. Someone else said that it would be 2 charges so am I wrong on this?? If >you did GMS on A1 and AFB on A2 then that would be 2 charges because its two >different stains. Tasha Campbell, B.S.,HTL(ASCP) Fr

[Histonet] new labeling system

2015-04-28 Thread Campbell, Tasha M.
Hello all, Does anyone know what we in the lab need to do for this new global labeling system for chemicals? Do we just need to get updated safety sheets from manufacturers for all chemicals in the lab? Do we need to change how anything is labeled in the lab? Any information is appreciated!

[Histonet] Need temporary Histotech

2015-04-23 Thread Campbell, Tasha M.
Hello everyone, I was wondering if there is anyone out there that does temporary work in histo labs in the Frederick MD area. I run a small GI lab and I will be going on maternity leave in October and we would like someone to fill in. I know that there are temp agencies I can go through, but I

[Histonet] List

2015-04-23 Thread Campbell, Tasha M.
Hello, I posted something to histonet and it said that I was a nonmember and it was awaiting approval but I have been a member for 2 years. Is something wrong with the server? Thanks. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301

[Histonet] staining racks

2015-03-23 Thread Campbell, Tasha M.
Does anyone have any Leica staining racks for the autostainer XL that they were sell/donate to me?? I just got a quote and its over $300 for 1 rack!!! That is crazy!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 14

[Histonet] temporary histotech position

2015-03-20 Thread Campbell, Tasha M.
Hello everyone, I was wondering if there is anyone out there that does temporary work in histo labs in the Frederick MD area. I run a small GI lab and I will be going on maternity leave in October and we would like someone to fill in. I know that there are temp agencies I can go through, but I

[Histonet] embedding

2015-01-21 Thread Campbell, Tasha M.
Hello everyone, I was just curious if anyone had tips on how to embed without getting paraffin on the outside of the cassettes so I don't have to scrape the blocks or at least not scrape very much. Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W.

[Histonet] Shurwave

2014-09-10 Thread Campbell, Tasha M.
Does anyone out there have a TBS shurwave microwave processor My temperature probe broke and I always keep an extra for when this happens. I have replaced them before. But this time I have replaced it with a new one and the temperature is still reading over 131C. Does anyone know what the p

[Histonet] PT

2014-08-13 Thread Campbell, Tasha M.
I need some help. I am a small GI lab in Maryland and need to find a proficiency testing program. I am only accredited by CLIA. I am only seeing things in the CLIA regulations and on other websites that just seems to focus on cytology PT. Does anyone know a program I can enroll in? Thanks.

RE: [Histonet] p16

2014-08-07 Thread Campbell, Tasha M.
Are you sure Dako has p16? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwe

RE: [Histonet] silver nitrate

2014-07-01 Thread Campbell, Tasha M.
11:23 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] silver nitrate You can rub salt on it and it should come up, I our salt on counter and make a thick scrub You can use Iodine and than wash with Sodum Thiosulfate Both work on hands the second

[Histonet] silver nitrate

2014-07-01 Thread Campbell, Tasha M.
So does anyone out there have a secret formula to get silver stain off counter tops? Thanks for you the help!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) __

RE: [Histonet] HTL

2014-05-21 Thread Campbell, Tasha M.
Definitely the booklets from NSH. Probably the most important, I think. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.

[Histonet] PT

2014-04-30 Thread Campbell, Tasha M.
I need to enroll in a Proficiency testing program just for the technical part. I am looking for the cheapest program that CLIA would recognize. I am only CLIA certified. Thanks. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD

RE: [Histonet] Re: Negative Controls

2014-04-30 Thread Campbell, Tasha M.
As of now, my lab took that as you just needed one negative slide per run. And that negative slide is getting the negative mouse or rabbit serum. Is this correct? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (

RE: [Histonet] Re: Negative Controls

2014-04-30 Thread Campbell, Tasha M.
So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every anti

[Histonet] GI Biopsies

2014-04-04 Thread Campbell, Tasha M.
Does anyone have problems with their GI biopsies kind of shredding when cutting? I microwave process them in the Shurwave microwave processor. I have been doing this for over a year and only the past couple months I have noticed that almost every biopsy I cut has knife lines in it. I have to chan

RE: [Histonet] CEU's

2014-03-12 Thread Campbell, Tasha M.
Leica has free webinars monthly. And Medpage Today has some free CEU's and also try FreeCME Weekly. I just google free CEU's and hope to find stuff. You can use the MLT stuff also, because it is a related field. You only need 2 points in the area which you are certified. And 1 point in lab or p

Re: [Histonet] GI medicare reimbursement rates

2014-02-20 Thread Campbell, Tasha M.
Medicare reimbursement is around $30 per biopsy. Sent from my iPhone > On Feb 20, 2014, at 4:42 PM, "Vincent Rivera" wrote: > > Hello my tried and true histonetters: > > I could use some help finding average reimbursement rates for the codes that > would be billed to GI specimens. Medicare

Re: [Histonet] Retic stain kits

2014-02-20 Thread Campbell, Tasha M.
Poly scientific is the best. You can try Mercedes medical. They are probably cheaper. Sent from my iPhone > On Feb 20, 2014, at 2:52 PM, "Piche, Jessica" wrote: > > Hello Everyone, > > Can anyone recommend a good retic staining kit that is reasonably priced and > works well? Thanks, > > Je

Re: [Histonet] Dried out liver bx's

2014-02-19 Thread Campbell, Tasha M.
Rough cut then soak in your waterbath for a minute or so then put back on ice to cool. Then section. U should get a beautiful ribbon :) Tasha Sent from my iPhone > On Feb 19, 2014, at 2:49 PM, "SARAH GIBSON" wrote: > > We are having problems with some of our small liver bx looking burned and

RE: [Histonet] Mineral Oil in Microwave Processing.

2014-02-17 Thread Campbell, Tasha M.
I have never heard of using mineral oil in microwave processing. I only use ethyl and isopropyl and paraffin. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: h

Re: [Histonet] Purple haze on H&E slides

2014-02-10 Thread Campbell, Tasha M.
What brand of reagents are u using and what is your protocol? What slides r u using and r u using gelatin in ur waterbath? Tasha Sent from my iPhone > On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC" > wrote: > > I am trying to get the histology lab up and running and I am having > diff

RE: [Histonet] Dako's Omnis

2014-01-30 Thread Campbell, Tasha M.
Are you talking about there IHC stainer, the Link? And there FISH kits or is there a new instrument that stains FISH? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message-

[Histonet] Microwave processing

2014-01-17 Thread Campbell, Tasha M.
Since I have been microwave processing GI biopsies, I have noticed that there is a blurry, fuzzy artifact on most of my slides in random spots on the tissue when looking under the scope. I asked the pathologist and he said he notices it too but he doesn't know what it is and it doesn't interfere w

[Histonet] RE: Warthin Starry Help

2014-01-15 Thread Campbell, Tasha M.
eilly [mailto:tony.rei...@health.qld.gov.au] Sent: Tuesday, January 14, 2014 7:38 PM To: Campbell, Tasha M.; histonet@lists.utsouthwestern.edu Subject: RE: Warthin Starry Help Hi Tasha I have been performing the Warthin-Starry successfully for nearly 40 years. Without seeing your method these are a few tips

[Histonet] RE: warthin starry problems

2014-01-14 Thread Campbell, Tasha M.
) From: Linda Margraf [mailto:linda.marg...@cookchildrens.org] Sent: Tuesday, January 14, 2014 11:12 AM To: 'histonet@lists.utsouthwestern.edu' Cc: Campbell, Tasha M. Subject: warthin starry problems Dear Histonetters: Here is a message I am posting from Tasha...she hasn&#x

RE: [Histonet] Warthin Starry Help

2014-01-14 Thread Campbell, Tasha M.
14, 2014 10:16 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry Help Hydroquinone has a short shelf life once opened. > On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M." wrote: > > I need help with warthin starry stain please.

[Histonet] Warthin Starry Help

2014-01-14 Thread Campbell, Tasha M.
I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first sta