Hello, I am a small GI lab and our facility is moving to a brand new building!
The building has yet to be built so I have full control on how to design my
lab. I will have about 1000-1500 sq ft. I was just wondering if anyone had
any tips for me? One thing I want to change is the type of flo
Can anyone help me out with the artifact I am getting on my slides? It is on
all of them, IHC, H&E, specials. It looks like formalin pigment, but I use
10%NBF so should I really be getting formalin pigment this much on my slides?
I am a GI lab. I use microwave processing. Specimens are held 2
-Original Message-
From: Eileen Akemi Allison [mailto:akemiat3...@gmail.com]
Sent: Friday, November 16, 2018 11:01 AM
To: Campbell, Tasha M.; Histonet
Subject: Re: [Histonet] test
** CAUTION: This email originated from outside of Frederick Memorial Hospital.
DO NOT click on links or
I keep trying to send an email for help with an issue I am having and its not
going through. It says the email has been sent but I am not getting the email
and no one has responded. I am testing to see if this message goes through.
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology As
Does anyone happen to have or know where to get HSV I and II and CMV control
blocks? The control slides are so expensive and it would be so much easier to
have a block that will last a long time and give me many for slides.
Thanks!!
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology
I am looking to install a small DI system or reverse osmosis system in my small
GI lab. We are bringing IHC in house and I thought it would be cheaper and
easier in the long run to have a DI system. I am not sure what type to get
though. There are so many out there. I don't want anything too
Is nuclear fast red the only counter stain for Prussian blue stain? I have a
Masson's trichrome kit and was wondering if the Bierbech Scarlet could be a
counterstain. I won't be doing the iron stain very often at all so I am trying
to keep dry reagents on hand to make up as needed so they do n
: WILLIAM DESALVO [mailto:wdesalvo@outlook.com]
Sent: Friday, July 27, 2018 1:20 PM
To: Campbell, Tasha M.; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Paperwork
I have a few suggestions:
Batch control - you do need to continue documentation of which cases/slides
corresponds to
Hi everyone,
I have 2 questions:
1. Could someone please share some ways to keep track of the control that
goes with the slides that it was used for? So I am a small GI lab and there is
a pathologist here a couple days a week. We do trichrome on Microscopic
colitis cases and so I have be
Hello all,
I am having issues with my trichrome stain and I am about to lose my mind! We
just started doing it in house (although at my previous job I had done
trichrome by hand for years so I am not a stranger to it. And I never had
issues with it). When I brought it in house at my current l
Hello everyone. I was wondering if any small, GI labs do IHC's in house and if
so what platforms you use. I am just researching right now to see if it would
be worth it to do in house or not. I know a lot of these instruments and
reagents are expensive so I would be looking for the most inexp
Anyone know who to contact to sell used equipment to? Thanks!!
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
h
Hi all,
Does anyone have any of the histomodules for the milestone RHS-1 110 cassette
rack they would like to sell? I need 3. I need the glass containers, lids,
stirrers, carriers, the disks and the heating disk.
Thanks!!
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associate
Hello all,
Is there anyone that can help me with my Shurwave Microwave Processor? The
unit is very slow reaching temp. I have to double the times of all steps for
it to get up to temperature. I have replaced both magnetrons, filament
transformers, high voltage transformers, capacitors, solid
Hello all,
I am looking for a histotechnologist in the Frederick, MD area that would be
interested in covering vacations and sick time for a small GI lab. Flexible
hours. Easy job. Please email or call if interested.
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310
Does anyone have a CLIA competency form for histology supervisor, histotechs,
and technical supervisors that they would be willing to share? It would be
greatly appreciated!!!
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800
I've been having this problem for a month or so. It is very humid in my lab
right now because of the weather here so I am very cautious about changing and
rotating everything everyday. But I am having issues with water being in the
first slide of the first run everyday! Its so weird! That i
Hello everyone, I was wondering if there is anyone out there that does
temporary work in histo labs in the Frederick MD area. I run a small GI lab
and I will be going on maternity leave in October and we would like someone to
fill in. I know that there are temp agencies I can go through, but I
>From my understanding its just one charge. Its per specimen such as A, B, C,
>etc. Someone else said that it would be 2 charges so am I wrong on this?? If
>you did GMS on A1 and AFB on A2 then that would be 2 charges because its two
>different stains.
Tasha Campbell, B.S.,HTL(ASCP)
Fr
Hello all,
Does anyone know what we in the lab need to do for this new global labeling
system for chemicals? Do we just need to get updated safety sheets from
manufacturers for all chemicals in the lab? Do we need to change how anything
is labeled in the lab? Any information is appreciated!
Hello everyone, I was wondering if there is anyone out there that does
temporary work in histo labs in the Frederick MD area. I run a small GI lab
and I will be going on maternity leave in October and we would like someone to
fill in. I know that there are temp agencies I can go through, but I
Hello, I posted something to histonet and it said that I was a nonmember and it
was awaiting approval but I have been a member for 2 years. Is something wrong
with the server?
Thanks.
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301
Does anyone have any Leica staining racks for the autostainer XL that they were
sell/donate to me?? I just got a quote and its over $300 for 1 rack!!! That
is crazy!!
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 14
Hello everyone, I was wondering if there is anyone out there that does
temporary work in histo labs in the Frederick MD area. I run a small GI lab
and I will be going on maternity leave in October and we would like someone to
fill in. I know that there are temp agencies I can go through, but I
Hello everyone, I was just curious if anyone had tips on how to embed
without getting paraffin on the outside of the cassettes so I don't have
to scrape the blocks or at least not scrape very much. Thanks!!
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W.
Does anyone out there have a TBS shurwave microwave processor My
temperature probe broke and I always keep an extra for when this
happens. I have replaced them before. But this time I have replaced it
with a new one and the temperature is still reading over 131C. Does
anyone know what the p
I need some help. I am a small GI lab in Maryland and need to find a
proficiency testing program. I am only accredited by CLIA. I am only
seeing things in the CLIA regulations and on other websites that just
seems to focus on cytology PT. Does anyone know a program I can enroll
in?
Thanks.
Are you sure Dako has p16?
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwe
11:23 AM
To: Campbell, Tasha M.
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] silver nitrate
You can rub salt on it and it should come up, I our salt on counter and
make a thick scrub
You can use Iodine and than wash with Sodum Thiosulfate
Both work on hands the second
So does anyone out there have a secret formula to get silver stain off
counter tops? Thanks for you the help!!
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)
__
Definitely the booklets from NSH. Probably the most important, I think.
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.
I need to enroll in a Proficiency testing program just for the technical
part. I am looking for the cheapest program that CLIA would recognize.
I am only CLIA certified.
Thanks.
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD
As of now, my lab took that as you just needed one negative slide per
run. And that negative slide is getting the negative mouse or rabbit
serum. Is this correct?
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (
So this has confused me more. So before you would run a negative control
for each block you were testing and you would use the negative mouse or
rabbit reagent. Now you don't have to do that but you still need a
negative tissue control. So what exactly does this mean? Does it mean
for every anti
Does anyone have problems with their GI biopsies kind of shredding when
cutting? I microwave process them in the Shurwave microwave processor.
I have been doing this for over a year and only the past couple months I
have noticed that almost every biopsy I cut has knife lines in it. I
have to chan
Leica has free webinars monthly. And Medpage Today has some free CEU's
and also try FreeCME Weekly. I just google free CEU's and hope to find
stuff. You can use the MLT stuff also, because it is a related field.
You only need 2 points in the area which you are certified. And 1 point
in lab or p
Medicare reimbursement is around $30 per biopsy.
Sent from my iPhone
> On Feb 20, 2014, at 4:42 PM, "Vincent Rivera" wrote:
>
> Hello my tried and true histonetters:
>
> I could use some help finding average reimbursement rates for the codes that
> would be billed to GI specimens. Medicare
Poly scientific is the best. You can try Mercedes medical. They are probably
cheaper.
Sent from my iPhone
> On Feb 20, 2014, at 2:52 PM, "Piche, Jessica" wrote:
>
> Hello Everyone,
>
> Can anyone recommend a good retic staining kit that is reasonably priced and
> works well? Thanks,
>
> Je
Rough cut then soak in your waterbath for a minute or so then put back on ice
to cool. Then section. U should get a beautiful ribbon :)
Tasha
Sent from my iPhone
> On Feb 19, 2014, at 2:49 PM, "SARAH GIBSON" wrote:
>
> We are having problems with some of our small liver bx looking burned and
I have never heard of using mineral oil in microwave processing. I only
use ethyl and isopropyl and paraffin.
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)
-Original Message-
From: h
What brand of reagents are u using and what is your protocol? What slides r u
using and r u using gelatin in ur waterbath?
Tasha
Sent from my iPhone
> On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC"
> wrote:
>
> I am trying to get the histology lab up and running and I am having
> diff
Are you talking about there IHC stainer, the Link? And there FISH kits
or is there a new instrument that stains FISH?
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)
-Original Message-
Since I have been microwave processing GI biopsies, I have noticed that
there is a blurry, fuzzy artifact on most of my slides in random spots
on the tissue when looking under the scope. I asked the pathologist and
he said he notices it too but he doesn't know what it is and it doesn't
interfere w
eilly [mailto:tony.rei...@health.qld.gov.au]
Sent: Tuesday, January 14, 2014 7:38 PM
To: Campbell, Tasha M.; histonet@lists.utsouthwestern.edu
Subject: RE: Warthin Starry Help
Hi Tasha
I have been performing the Warthin-Starry successfully for nearly 40 years.
Without seeing your method these are a few tips
)
From: Linda Margraf [mailto:linda.marg...@cookchildrens.org]
Sent: Tuesday, January 14, 2014 11:12 AM
To: 'histonet@lists.utsouthwestern.edu'
Cc: Campbell, Tasha M.
Subject: warthin starry problems
Dear Histonetters:
Here is a message I am posting from Tasha...she hasn
14, 2014 10:16 AM
To: Campbell, Tasha M.
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Warthin Starry Help
Hydroquinone has a short shelf life once opened.
> On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M."
wrote:
>
> I need help with warthin starry stain please.
I need help with warthin starry stain please. I am a very small lab
trying to save money so I want to make the reagents myself. I have
sigma Aldrich dry reagents and have been making the reagents and
following the instructions correctly and I did get the stain to work
wonderfully when I first sta
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