[Histonet] H&E staining

Charles,extend time on the deparaffinization station,and even better,renew solvents at this point also. My kind regards, Carlos Defeo Histotechnologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/l

[Histonet] nuclear bubbling

Dear James: Two chained events take place on your issue: 1-sections not entirely drained,water remains even minimally under sections; 2- you put these sections in the oven, the heat literally "explodes" the nuclear bubbles and creates a hole with no cromatin to stain. My kind regards, Carlos D

[Histonet] Thick and thin sections

Tyrone: definitely no temperature issue in this case. Look if your using cassete that is correctly clamped, and then if the answer is no, check the other parts of the blade holder unit. If still no part seems to be loosen, you have to service the micrometric advance, what seems to be the most re

[Histonet] Best solution for surface decalcification of paraffin block

Hi all, I would like your opinion on the best way to achieve many few decent sections of calcified material. IED by Biocare,a strong acid solution, any input would be greatly appreciated. Thanks in advance. Carlos Defeo Histotechnologist ___ Histonet m

Re: [Histonet] IHC hand staining

IHC staining for H.Pylori could be done using monoclonals from CellMarque or Biocare, with a simple protocol reccommended by the vendor, My regards, Carlos Histotochnologist -- Mensaje original -- De: "Elaine allison Hoffman via Histonet" Para: "Histonet List" Enviado: 05/11/2015 13