[Histonet] Plastic embedding and cutting training (Charles Riley)

Hi Charles, I found this. There may be more on Google Scholar using a more specific search. Plastic Embedding and Sectioning of Xenopus laevis Embryos YouTube *

[Histonet] Unstained slides precut for IHC

on edge for IHC? Additionally, when sending slides for IHC to other labs is it preferred to send dry slides or baked slides? Thanks for your input! Carrie Disbrow, HTL Get Outlook for Android<https://aka.ms/AAb9ysg> ___ Histonet mailing list Hi

[Histonet] Autopsy blocks and slides

Autopsy per CAP is ten years block storage for both regular and forensic. Forensic slides must be kept thirty years. Found that info with a Google search. Get Outlook for Android ___ Histonet mailing list Histonet@lists.utsouthwe

[Histonet] Congo Red Controls

Hi,A pathologist told me to try medullary thyroid carcinoma cases for Congo Red. Cheaper than purchasing if positive.Good luck! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwe

[Histonet] Immuno controls

Hi all, After consideration I disagree with the consensus and also agree with Curt's pathologist. More supplies and labor will be involved as cutting more controls will be neccessay to meet the requests of the pathologist. The patient tissue is put on two controls slides. One slide is used

[Histonet] Labeling issues

Hi All, We are writing more numbers as the year goes. S10-26,262 A1 - L1, has a combination of 12 numbers and letters with a hyphen and comma. Only three more numbers compared to the beginning of the year with S10-126 A1 - L1 but the potential for more mistakes. And what about the ergo

[Histonet] The Fifth Annual International Retreat on Applied Immunohistochemistry and Molecular Morphology

rate for early registration and I would like to complete the process soon. Thanks for any info, Carrie Disbrow, BS, RVT, HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Posting comments

As a newbie trying to learn as much as possible, I'm disappointed when questions are posted and the responses are sent to the individual. I learn more when the responses are shared. Also, an archive search on the subject will have limited information. I would think there are many in the sam

[Histonet] dog with tail lacerations

Hi, You might want to access IVIS, a compilation of veterinary presentations from meetings and journal articles. It is free. You can then do a search for "lick granulomas", tail injuries, etc. I know of cases where the dog has been sleeping next to the door on the inside and the owner opens th

RE: [Histonet] HTL exam

stonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Wednesday, April 14, 2010 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL exam Hi. I'll be taking the ASCP HTL exam in three months. Does anyone know what

RE: [Histonet] HTL exam

Safety Officer LRGHealthcare 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Wednesday, April 14, 2010 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [

Re: [Histonet] list responses

Hi Adam, thanks for responding so quickly. Carrie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HTL exam

Hi. I'll be taking the ASCP HTL exam in three months. Does anyone know what the percentage of enzyme histochemistry, electron microscopy, and cytology questions are for the HTL? Thanks, Carrie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] list responses

Hi We kept wondering why our responses to the discussion are not seen on the daily list. We then realized the response must be going directly to the person who wrote the original post. Is there anyway the responses can be seen on the list as we are losing the input of the others. Thanks, Carrie

[Histonet] amyloid ? for Bob Richmond

Hi, I was also told or read somewhere that insulinomas are good amyloid controls. I've never had a chance to test any blocks from these cases. What has your experience been? Thanks for your help. Carrie ___ Histonet mailing list Histonet@lists.utsou

[Histonet] antibody validation

HI Everyone and Happy Easter! Just wondering how you validate your antibodies? I've read that some antibodies use negative controls with the genes or proteins removed and that the positive controls have the protein added. Is that the procedure for research or manufacturing situations? Thanks, Ca

[Histonet] More Copper stain thickness

Hi Histonet! Regarding the discussion about the thickness of FFPE sections for Rhodanine copper, ie 3 um vs. 6 um, someone asked me: If everyone agreed that thicker sections are better, how it can be a 50/50 split about the thickness? Isn't that an unsustainable contradiction? (and I thank them

[Histonet] Re: Responses to micron thickness for Rhodanine copper

Hi and thank you all for your responses! Everyone agreed that the thicker sections allow the copper pigments to be better visualized. However the responders were split 50/50 in the thickness. There were labs that did cut the sections at 3 microns with continuous good results and the others at 6

[Histonet] Rhodanine Copper stain

the block again. Thanks for your input. Thank you, Carrie Disbrow, HT ASCP (soon to be BS, HTL ASCP :-)) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] ISH and Bone Marrows question

Hi Histonetters! We have recently changed to Immunocal to decal bone marrow biopsies. Immuno stains performed on the decaled bone marrows look good. All In-situ hybridization negative controls, and Kappa and Lambda stains on decaled bone marrows have a blue artifact at the edges. We use the V

[Histonet] Hi Jan,

Hi Jan, Thanks for your input! I have two A. S. degrees. One in veterinary nursing/technology and one in histology. And I have an AA where all my electives were biology,chemistry and micro. Then I'll have a BS in veterinary nursing/management. I'm starting a molecular program in January. So, I

[Histonet] Hi.

Hi. Hearing all these comments about histology is really depressing. I was hoping by getting my BS and upgrading my ASCP certification I would at least get, what I consider, a more interesting job. My BS will be veterinary technology, similar to a registered nurse with a bachelors but for anima

[Histonet] What percent of HTL do not have a BS?

Hi! The bigger question is are there better jobs available for HTL's with or without a BS. Also, I thought ASCP required the BS for the HTL certification. I, for one, have been working very hard to get my BS so I can upgrade my certification to HTL. Many of the people in the lab I'm in have thei

[Histonet] Hi Tony,

Hi Tony, Thanks for the info. I understand the discussion topic. I just wish their was one straight answer! But then this is histology! Carrie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/

[Histonet] PO/Zylene on cytospins for Fites

Hi Rene, Thanks for the info. We are doing a AFB Fites for Nocardia. There is filamentous bacteria in the patient GMS and the docs are differentiating the type. I did not deparaffinize the patient cytospin slides and I did deparaffinize the patient control with Z/PO. The problem I had been anti

[Histonet] AFB Fites of cytospins

Hello Histonet. Has anyone had experience and good results doing AFB Fites on cytospin slides from BAL's? Do you use the peanut oil/xylene (PO/Z) solution or just 100% peanut oil? Or does the cytospin slide need any peanut oil? After the deparaffinization step, if it can be called that, we are go

[Histonet] dako artisan gram and afb

Happy Holidays to all. W ea re using the Dako Artisan stainer and have had problems lately with the AFB and Gram staining inconsistently from day to day. We have had the machine services and the PM done and the problem has returned. Has anyone had the same problem and what did you do? Thanks fo