Good afternoon, I am currently working with Lewis rats performing corneal alkali injuries at varying strengths. Is there someone there that has prior experience working with a rat eye and would be willing to share information on the most effective ways to preserve, fix and cut the cornea sample.
We are interested in using the cornea without using the whole globe if possible. For now we will be using basic H&E staining with a possibility of immunohistochemistry at a later time. The main outcome we are looking for is to find the presence of neutrophils in the cornea. A second objective is to look for any damaged or newly reconstructed tissue. I would greatly appreciate any advice relating to the type of paraffin used, the ideal length of time to save the tissue and any assistance you can suggest for completing this process successfully. Thank you for your time. Any assistance will be greatly appreciated. Sincerely, Casie Phillips casie4...@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet