Hello Histology friends,
We are a research lab with a 5 year old Leica ST5020 autostainer that looks
like new. We run less than 5000 slides a year on it. We noticed that (of
course just after the warranty ended) that one of the water rinse stations
stayed full of water all the time, even when
Hello Histo-friends,
Is anyone doing a TUNEL stain on any of the Leica Bond instruments? I will be
attempting to set this up and thought I would reach out first.
Thank you,
Cathy
Cathy M. Mathis
Laboratory Technician IV
-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
Hello Dr. Rosen and Histonetters,
We have used a Leica Bond RX for over 2 years now, cleaning and caring for it
just as you do. My work is mostly animal which is why we mainly choose the red
chromogen alkaline phosphatase detection kit. We see the same artifact
sporadically. Sometimes we will
Hello Histo-netters!
I have a Bond RX and before I try to recreate the wheel, I thought I would ask
some experts. Has anyone used a goat primary on this instrument and if so how?
Also, has anyone had success with TUNEL on this instrument and if so, could
you share your knowledge?
Thank you all
This is a great website for NHP info. Thank you all for all your wonderful
help!!
Hope everyone has a great 4th of July weekend
Cathy
-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
[mailto:histonet-requ...@lists.utsouthwestern.edu]
Sent: Friday, July 01, 2016 1:
Rhesus is a species of old world monkey. There are a couple of other companies
that have this same clone and on their datasheets they say it cross reacts with
rhesus monkey. The CD34s (2) that I have do not state on their data sheets
that they cross react with anything except human and yet are
2 more bits of information about my dilemma;
I did try staining without any retrieval - no signal
I am using rhesus kidney as a control (getting no signal), but I also ran some
human kidney and got beautiful staining with a 20 minute HIER using a pH 8 EDTA
buffer.
So I know the antibodies and my
Good morning my fellow Histo-netters,
Does anyone have experience with any CD34 antibody that would work in FFPE
rhesus tissues? A few companies have one with clone QEBend 10 and say that it
works but I have had no luck. I am using the Bond RX polymer system and I've
tried all the epitope retr
