[Histonet] Congo Red filters

Fran - You can use Whatman #1 filter paper but it is a lot cheaper and just as good to use 2 coffee filters. They are the right size for filtering stains. Cheryl Crowder ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Excell Plus

Hello - has anyone used the non-formalin fixative Excell Plus? I have a researcher who wants to use it. I'm particularly interested in it's dehydration ability since it is mostly alcohol based. Thank you. Cheryl Crowder LSU Ag Center __

[Histonet] Mycoplasma replies

I want to thank those who responded to my inquiry about staining mycoplasma. The researcher has access to DiffQuik stains in particular and is going to.try that first. Wish us luck.z Cheryl Cprowder ___ Histonet mailing list Histonet@lists.utsouthweste

[Histonet] Mycoplasma staining

I work with a researcher who has mycoplasma species cells on agar. He does not have access to a fluorescent microscope. Does anyone know of a technique using regular stain? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsout

[Histonet] Giemsa control

Charles - The best control for a non-blood Giemsa stain is a mast cell tumor from a canine. You can probably get a block from the Univ of Penn Vet School. It is really close to you. The mast cells will stain purple or magenta. Cheryl ___ Histonet mai

[Histonet] marking tiny specimens

dyeing or marking these tissues so I can see them better to embed. Thanks in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] India ink

ink is permanent there was no problem losing it during processing and the fine points left just small dots. The only thing is that the tissues should be blotted well as moisture seems to cause a little bleeding. Cheryl Crowder, HTL(ASCP

[Histonet] Cresyl echt violet

Some years ago the name of cresyl echt violet was changed to cresyl violet acetate (no CI #). It is what is now used in staining for Nissl substance. Cheryl Crowder ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Used tissue processors

Hi - Would the person who contacted me about a used processor please e-mail again. Thank you. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 ___ Histonet mailing

[Histonet] Horse eye fixation

er initial fixation with Davidson's (or modified Davidson's), place the eye in 70% alcohol overnight. There is no need to place the eye in formalin after the Davidson's fixation. Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, L

[Histonet] Need parts for old equipment

ectronic switch. Any help any one of you can give me, contact me personally. Thank you in advance, Cheryl Crowder, BA, HTL(ASCP) Animal Science Department LSU Baton Rouge, LA 70820 (225) 772-2865 ___ Histonet mailin

[Histonet] Light green for PAS

ink of the PAS. Cheryl Crowder ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] EM question

I am the first to admit I know little to nothing about EM. I have a researcher who read an article today that said in the method that they had taken cells, applied them to copper EM grids and immersed them in liquid nitrogen to fix them. Has anyone ever heard of this technique? and if you hav

[Histonet] Toluidine Blue

Henk - Mast cells, on tissue and/or cytospins, are pH dependent - 2.0-2.5. Tissues are usually already fixed in formalin; cytospins can be fixed in 95% alcohol or formalin. I am sending our directions separately. The stain should less than 5 minutes totally. Cheryl _

[Histonet] Bouin's without picric acid

ours. But many of the fish we get have been fixed for a week or more and results are really good. Contact me personally if you need more information. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225)

[Histonet] Grimelius stain

Sara- We stained many tissues for chromogranin and found that the Churukian stain worked a lot better than the Grimelius. It double impregnates the cells with the silver. Directions are in Carson's book, but if you don't have it, I can send them to you. Cheryl Cheryl Crowde

[Histonet] Sections sticking

Bret - You can also check the back side of your blade. We wipe the front of the blade often, but forget about the back. If paraffin builds up on the blade the sections will stick. Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Bile counterstain

Kim - You can use eosin lightly to counterstain the bile stain. The green will still contrast well. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 ___ Histonet

[Histonet] What we do for a living

Hi - The NSH has a terrific coloring book what explains what we do and is really fun. It's meant for kids but I used to give it to "newbies" in our area. It cost a dollar I think. But whatever price, it's worth it. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histo

[Histonet] Fwd: GMS counterstain

Ally - I have worked with several pathologists who were color-blind -one to greens; one to blues. As a result we counterstained the GMS with different colors. A counterstain is just that. It gives a color to secondary tissue elements. Light greens, hematoxylin, metanil yellow and nuclear fa

[Histonet] Thionin staining

An - You didn't mention what cells you were staining, but I assume it's mast cells. Thionin stains are very pH dependent. For mast cells, the pH should be 1. At that pH only mast cells stain. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark

[Histonet] Animal tissue

If you are processing mammalian tissue, it can be run with your regular "human' tissues. Rodent tissues are totally different and require shorter processing times. As long as your animal tissues are grossed at the proper thickness you should have no trouble. Cheryl _

[Histonet] Dyes and chemicals

the dye for a long time. Chemicals, on the other hand, can outdate. Your best bet is to dispose of them properly. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865

[Histonet] Copper Stain

Pamela - We stained copper with Uzman's method, manually. It uses rubeanic acid (dithiooxamide) solution. The original procedure takes 2 days, but we were able to modify it to take less time. I can send it to you personally if you want to try it. Copper shows as a fine black precipitate.

[Histonet] Decal solution

Hi - There are a myriad of decal solutions. You could contact Gayle Callis - she is an expert and has given workshops on bone and decaling for some time. We have used her suggestions of 15-20% formic acid for years, with no detrimental outcomes even for IHC. However, if you don't want to make

[Histonet] AFB

Amy - The AFB (Ziehl-Neelsen, Kinyoun's, etc) will stain any acid-fast bacteria. The Fite's stain is usually considered specific for mycobacteria. Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailm

[Histonet] Nitroblue Tetrazolium Chloride (NBTC)

Laurie - Tru Sigma/Aldrich. I used to get mine from them. Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] slide staining racks

Hi - I am staining by hand for a research project and am in need of some 30 slide staining racks. If anyone has any that they want to get rid of, please contact me. I can use multiples so, even if you have only one. Cheryl ___ Histonet mailing list

[Histonet] Vacuoles

Nareen - Vacuoles in brain and rat testes are often caused by the block being too cold and dry. Try facing off the block and then placing on melting ice or cold water. This will rehydrate the block and raise the temperature. Also, cut slowly, particularly the brain. Vacuoles seen to appear

[Histonet] Reticulum stain wash-offs

Rather than use Halt (or other adhesive) in your water bath, have you tried using charged slides - those used for IHC. Then air-dry or heat dry the tissues before deparaffinizing. Any water left in the tissue will cause wash-offs. Do not lower the pH of the silver solution. Cheryl Crowder

[Histonet] Tilapia eyes

processed properly, you have a better chance of getting a good section. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FA

[Histonet] Direction for TP 800

Hi - Got a taker for the directions. Who know? Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720

[Histonet] A.O TP8000 Processor

Hi - I'm cleaning out my office and found the procedure manual, timing disc and notes for the old A/O TP8000 Processor.If for some reason someone would like these I will mail them to you. Otherwise they are going in the trash. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technol

[Histonet] Fish Gills

don't cut well, after facing off soak them in Downy for an hour or so. The first sections should be really good. If you have any questions, contact me directly. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of

[Histonet] Slide labeler

it's a hassle. Also the ink and light bulb are expensive, but we wouldn't give ours up for anything. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bert

[Histonet] Embryo fixation

Thank all of you who answered by question about fixation of chicken embryos. We have decided to try alcoholic formalin on the early embryos and add acetic acid to the older ones with calcification of the calvarium. Wish us luck. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist

[Histonet] Fixation of embryos

really appreciated. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720

[Histonet] Chromium trioxide

You can use periodic acid to oxidize your tissues as you would for the PAMS stain. However, this is not the optimal solution when staining fungi. 5% chromium trioxide used for the GMS can be reused. Since you are doing the stains manually, as many of us do, this works particularly well. The

[Histonet] Toluidine Blue stain

Jen - We do T=blue staining almost daily. If you will contact me directly I will send you our directions. It is a very inexpensive stain, and can be reused. You will need to pH it often. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of

[Histonet] 2009 Louisiana Society for Histotechnology 26th Annual Symposium/Convention

Hello Histonetters, The Louisiana Society for Histotechnology is pleased to announce the 26th Annual Symposium/Convention: "Your Histeaux Surplus Package" June 12 & 13, 2009 at the Bourbon Orleans Hotel 717 Orleans St. New Orleans, LA 70117 www.bourbonorleans.com The LSH block of r

[Histonet] Epithelial cells

Hi all - We have a graduate student growing equine epithelial cells in culture. Money is really short. She is wondering if there is a regular special stain for these epithelial cells rather than the more expensive IHC. Any suggestions? Thank you, Cheryl Cheryl Crowder, BA, HTL(ASCP

RE: [Histonet] Sakura tape problem

Thanks for the tip. All I'd need to do is lose the tissue too. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-973

[Histonet] Sakura tape problems

Thanks to all of you who have had problems with the Sakura tape. I have received multiple methods to try to save the sections. When I have tried several and combined some I will let you all know what happened so maybe I can help you. Thanks again, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief

[Histonet] Sakura tape problem

can it? The pathologists are really upset over these slides as they are for continuing education and cannot be replaced. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University

[Histonet] section thickness

microns. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720

[Histonet] softening decals

"scent" of the softener was too sweet and we hated smelling it. We chose another scent for the next container. I have no idea why it works, but it does. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinar

[Histonet] Colored paraffin

tissue. So it's a double edge sword. If we used white cassettes all the time, the colored paraffin would be great. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University

[Histonet] Re: Histonet Digest, Vol 58, Issue 8

Maxim - You sent me an e-mail in August. I replied several times, but the e-mail did not go through. Is your address complete or is there another I can use? Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary