w the desired blueing. Alternatively, substitute TRIS
or similar as the buffer in the preceding step and go directly to tapwater.
If you have valuable sections with crystals on them, you should be able to
chelate away the deposits in an EDTA solution, then restain as needed.
all the best!
-Dav
sion. You could check out some of
their muscle to see if it is of a suitable quality.
I'd ask the veterinarians in your animal facility to suggest someone who is
doing this regularly, since this does require institutional approval as a
non-survival surgery under deep anesthesia.
-David
==
D
endogenous peroxidase in eosinophils
Anyone have tips for quenching endogenous peroxidase in eosinophils? Our
standard px block is not doing the job (Biocare Peroxidazed-1).
Thanks,
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
========
David A. Wright, Ph.D.
University of Chicago
Section of Ne
r else you will neutralize
your primary with the residue.
Sorry this isn't a specific protocol, but I haven't seen any other reply (I
only read the daily digest)
-David
==
David A. Wright, Ph.D.
University of Chicago
Section of Neurosurgery, MC3026
>Message: 1
>Date: Thu, 1 Dec 20
chromogen
(and, if you do more Ag retrieval, you would remove accessible complexes that
way). I do sequential peroxidase reactions routinely, with no x-rxn after DAB
1st round.
I hope you were not planning to re-stain for the same antigen; it will be
masked by the DAB ppt.
-David
==
David A. Wri
rate from both sides of the retina - extra
valuable because your incubation times are going to be very long with material
this thick. (I assume you are not just interested in superficial layers.) A
microwave processor would help with penetration, but I have no direct
experience.
-David
==
D
needs 4x the time.
Note that the same principle applies to leaching out unbound
reagents - you should lengthen your wash steps too, by the
same factor, to prevent high background.
happy staining
-David
David A. Wright, PhD
University of Chicago Section of Neurosurgery
Original message
Da
lot
even with good stuff (approx every half liter).
-David
------
David A. Wright PhD
University of Chicago Section of Neurosurgery
Original message
>Date: Thu, 11 Sep 2008 12:09:50 -0500 (CDT)
>From: [EMAIL PROTECTED]
>Subject: Histonet Digest, Vol 58, Issue 13 Message: 1
>
e B44) which only needed a few minutes
in 0.07N NaOH to unmask to BrdU. I think it's still available.
Good luck!
-David
David A. Wright PhD
University of Chicago Section of Neurosurgery
Original message
Histonet Digest, Vol 58, Issue 13 Message: 11
Date: Thu, 11 Sep 2008 1
