Re: [Histonet] DAB IHC with H & E staining

2016-01-06 Thread David Wright via Histonet
Hi Histonet, Marcus and Liz I agree with everything Liz says about precipitated DAB being wonderfully robust so that you can do a whole range of stains successfully after using it. I am slightly puzzled that Marcus wants to reveal RBCs after a DAB reaction. Doesn't it just happen any way? Haem/

Re: [Histonet] failure of nuclear counterstaining after IHC

2016-01-04 Thread David Wright via Histonet
Hi Histonet & Maria Is it possible that Maria really means Nissl staining of neuronal cell bodies (somata) together with their nuclei, rather than just nuclei? Tim is right to point out that the staining target is likely getting washed away during IHC processing, but I suggest it is Nissl subst