Hello all,
I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5.
I have tried in vain to get this to work but regardless of the order of
staining the end result is dark blue/purple mucin. I have carried out a
parallel experiment whereby the staining has been checke
Sharon,
There are little springs located behind the knife holder plate . If one of
these is lost then it can cause the twisting that you describe. This happened
to me years ago when stripping and cleaning one of our Leica 2035 microtome's.
Hope this helps.
Debbie
Debbie Faichney
Histopathol
Hi all,
I have a Masters student here who has Horseshoe crab tissues fixed in 10% NBF.
Most of the tissues have been sectioned successfully but the eyes are
surrounded by armour plated chitin! (she broke two Dremel drills trying to cut
the carapace and finally tin snips had to be used to cut t
Hi Ana,
Yes, I work for an aquaculture veterinary pathology lab and have experience
with some of these, but particularly fish.
Please feel free to contact me for more information, I'll help if I can.
Debbie Faichney
Histopathology
Institute of aquaculture
University of Stirling
Stirling
Scotland
