There is a fantastic resource for antibody assessment and optimization and they
have done p16 as well as many many other antibodies. Their results of testing
is free on line. p16 is http://www.nordiqc.org/Epitopes/p16/p16.htm from 2009
with many different companies antibodies.
Here is the lis
Available through VWR, but may be cheaper elsewhere. They work much better
than I expected.
ZEROSTAT ANTISTATIC GUN VWR cat #100496-120 Vendor cat #60610
Each$122.64 list
Donna Harclerode, HTL, QIHC, SLS (ASCP)
Associate Scientist
Celgene
9393 Towne Center Road
San Diego,
I had major problems with frozen trichrome years ago and Jamie Watson from GNF,
La Jolla said to fix in the Bouins and not fix in formalin and then mordant in
Bouins. (duh) I am not sure why I never tried that, but when I fixed in Bouins
for 10 minutes I got consistent trichrome on frozen skin s
There was some discussion last week and I dug up the info on my favorite chairs.
I purchased some excellent chairs at a very reasonable cost from eStores that
sells chairs from Office Master in Ontario, CA. The chairs come in all kinds
of fabric including the lab vinyl. The distributor's site i
Hi Alexia
I am not sure exactly what you are describing but my first question would be
what is your nuclear stain in? If it is an alcoholic solution the sections may
get shocked and stick together. I normally used DAPI in the secondary to
counter-stain nuclei which is aqueous so that was less s
I have done flat retina sections- which may be what you are being asked to do
which is a very specialized procedure even for most research labs.
Do you have access to a sliding microtome with a freezing stage? Otherwise I
do not know any way to do this. If you are doing flat retina (I have done
I have a tiny project using araldite on mouse nerves and wondered if anyone
could recommend the tungsten carbide knife that Ted Pella cat #121-50 or any
other company sells. I cut methacrylate ages ago with glass knives but wonder
if the tungesten carbide blade might be a easier and way less ex
You can use glass camera filters. At the store they had a huge supply of
polarizing filters. I bought 2 for about $20 each that were the same brand,
but not even the same diameter. I put one on top of the slide and one on the
light source and it worked great. I also rotated the light source
The San Diego chapter will be having our next meeting on Sept 10 from 4:30 to 7
at Pfizer. The topic will be on laboratory safety and hopefull we will come up
with more topics for future meetings. There will be a light dinner and
everyone is welcome but please let us know you are planning to a
One more possible issue along with the others. Over tightening of some
disposable blades can cause thick and thin sections. Leica 1800 and 3050 only
should have the knife tightened snug, not have the handle (tightening bar or
whatever you call it) pushed as far as it goes. My rule of thumb is
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