??
>
>
>
> Cheryl Kerry, HT(ASCP)
> Full Staff Inc.
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they are guaranteed for 1 year that they will break down after that time
and then we will play the replacement rodeo on our dime.
Thank you so much for your help!
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She/Her
Histology Supervisor
Tempus Labs
600 W. Chicago Ave.
Chicago IL 60654
Cell: (708)269
, how
reliable you find the instrumentation to be, and how user friendly it is.
Additionally, does it require a lot of maintenance?
Thank you all - feel free to DM me if you prefer.
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Histology Supervisor
Tempus Labs
600 W. Chicago Ave.
Chicago IL 60654
Cell: (708)269
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Chicago IL 60654
Cell: (708)269-8610
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rences one way or another. Does anyone have
any policies regarding this?
Thanks so much!
Erin Martin, Histology Supervisor
UCSF Dermatopathology & Oral Pathology Service
Phone: 415-3537248 | Fax: 415-353-7543
CONFIDENTIALITY NOTICE: This email message, including any attachments, is for
t
ce we are usually
embedding for 4 to 5 hours the ability to change positions would be nice.
So my suggestion would be a get a higher table so that you could sit or stand
depending on your mood!
Have a great Wednesday!
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology and Oral Path
> UT Southwestern
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> The future of medicine, today.
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this for samples
that in the past have had large quantities of recuts.
Thank you for any information you can provide!
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Histology Supervisor
Tempus Labs
600 W. Chicago Ave.
Chicago IL 60654
Cell: (708)269-8610
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Histology Super
f my ability to solve.
Thank you in advance for any advice or consulting recommendations!
Have a lovely Wednesday!
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology and Oral Pathology Service
1701 Divisadero Street, RM 230
San Francisco, CA 94115
415-353-7248
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showing
negative results.
I currently am using the Ultraview kit for optimization, our pathologist
likes CC2 for 44 min, Ab. Inc for 40 min using a 1:100 dilution.
Anyone have any suggestions that might help? If you want more detailed info
I can provide as well!
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HSRL (Histo-Scientific Research Labs) is a leading GLP contract research
organization which specializes in veterinary histopathology since 1999. Our
staff has been committed to scientific integrity and superior
responsiveness to client needs. A career with HSRL offers you potential to
join an estab
right over to the Benchmark
so I can see why the idea that it can run our IVD items seems plausable.
However what I need to know is which is correct?
I do not want to do something that I am uncomfortable doing without
checking with others in my field first.
Thanks!
--
Erin McCarthy
Hi All,
When cutting for NGS testing what additional measures do you take to
minimize contamination? We are a pretty clean outfit here, but still, have
some trace sample carryover occurring in cases we cut in-house. We already
use a fresh blade with every case, use tools like forceps instead of
fi
back
to the original facilities. Any questions I will gladly add more!
Thanks!
--
Erin McCarthy, HT (ASCP)
Lead Histotechnician
Tempus Labs
600 W. Chicago Ave.
Chicago IL 60654
Ph:(312) 638-6344 Ext.3835
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hard to
access what a person is capable of, would it be acceptable for me to have
applicants performs a cutting time test?
I am open to hiring new techs, but I want to make sure we are not hamstrung
by a great interviewer with a lacking technical skill set.
Thanks!
--
Erin McCarthy, HT (ASCP
Good morning histoland!
Has anyone had success with the ROS1 antibody from CellSignaling? I just can't
seem to get it working beyond what our pathologist call "a faint blush". I
would greatly appreciate if you would be wiling to share your protocol!
Have a lovely Monda
going out with repetitive motion injuries
because they ended up with an unfair portion of the workload. Thank you in
advance for any suggestions!
Erin Martin, Histology Supervisor
UCSF Dermatopathology and Oral Pathology Service
1701 Divisadero St, San Francisco, CA 94044
415-353-7248
Confidentia
first few photos
seen here from Ted Pella:
metal microscope slide racks
<https://www.google.com/search?q=metal+microscope+slide+racks&client=safari&rls=en&source=lnms&tbm=isch&sa=X&ved=0ahUKEwjRrLHwqafVAhUB4SYKHa0OA9QQ_AUICygC&biw=1240&bih=625#imgrc=_>
Thanks in
out. Thanks in advance for
your help!
Regards,
Erin
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Good morning histonetters!
I'm looking for a new source for HHV-8 (KSV), clone 13B10, antibody.
CellMarque's is no longer IVD, nor is Ventana. Does anyone have a suggestion?
Thank you!
Erin Martin, Histology Supervisor
UCSF Dermatopathology and Oral Pathology Service
1701 Div
Hi all,
Does anyone know of a source for a Leishmaniasis control block or control
slides for IHC?
Thanks in advance!
Erin Martin, Histology Supervisor
UCSF Dermatopathology and Oral Pathology Service
1701 Divisadero St, San Francisco, CA 94044
415-353-7248
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Good morning everyone!
One of my pathologists says that we are having a problem with the tissue on the
slides looking vacuolated and torn. He is convinced it is from microtomy.
Anyone have any ideas? I was thinking that it might be a processing issue.
Thanks in advance!
Erin Martin
I am willing to purchase and pay for shipping if anyone has any they are
getting rid of. Thank you!
Erin
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Hi all,
I am going to take a shot in the dark here and see if anyone has a block
holder/chuck for a microm microtome that they would be willing to sell. Thanks
in advance for your help!
Regards,
Erin
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Hi all!
Santa Cruz Biotech has to discontinue all goat and rabbit antibody production:
http://blogs.sciencemag.org/pipeline/archives/2016/05/23/trouble-at-santa-cruz-biotechnology
I hope you are all having a great Tuesday!
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology
Hi Histonet-
I am searching for a block holder for our Microm microtome and am wondering
if anyone has one they are willing to part with or sell. Thanks in advance
for your help!
Erin
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prefer HR and LR be run on separate slides instead of as a cocktail.
Anyone have any thoughts?
*Erin McCarthy HT, ASCP*
Third Shift Lead Histotechnician
OSF Saint Francis Medical Center
530 NE Glen Oak Ave. Peoria, IL
Phone (309)624-9043
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crystals and that it
would be difficult to get the sections to stick. If you have any advice I
would greatly appreciate it!
Thanks,
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology and Oral Pathology Service
1701 Divisadero St, San Francisco, CA 94044
415-353-7248
Hi Histonet-
Does anyone have any slide racks that hold the slides horizontally that
they no longer need? Like this but for maybe 50-60 slides?
http://grale.com.au/products/view/297
I am willing to purchase and pay for shipping if anyone has any they are
getting rid of. Thank you!
Erin
Was wondering if anyone else is experiencing any h&e staining issues recently
using the symphony stainer? Our lab has been experiencing uneven staining and
dark mucin staining especially on our Gi biopsies. This has been happening on
specimens that have been processed on different processors, tw
th it. That's why there are often issues with film coverslipped slides from
that time period. It's a pain to deal with but not difficult.
Good luck!
Erin Martin
UCSF Dermatopathology and Oral Pathology Service
Message: 5
>On Tue, 10 Mar 2015 John Kiernan jkier...@uwo.ca<m
Histology Laboratory located in the Shenandoah Valley of Virginia is
looking to add to it's team. In this position, you will need a working
knowledge of IHC theory and practical IHC experience. The best candidate
for the position will oversee immunohistochemical staining as well as
perform other hi
around $400, so I
wanted to reach out to histonet to see if anyone had any other options.
Thanks for your help and have a great day!
Erin
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Good morning! I agree with some of the other comments - "per day" is too
variable because of tissue type, overall volume, etc. We use hourly average to
try to keep everyone in the same range but still leave room for individual
abilities.
Erin
Erin Martin, Histology Super
Good morning all! Would anyone mind sharing their plans for power failure? We
have backup generator power on red plugs but my pathologists want a plan for
what we would do if the power went out AND the backup generator failed.
Thanks for your help!
Erin
Erin Martin, Histology Supervisor
a few paper towels on the foam
before you put the specimen down to pin - it will allow the formalin to get
under the tissue. Foam can be a bit crumbly, like cork, but since you are
repurposing something that would have gone in the trash you can always throw
them out after the specimen is fixed.
every 3
months per Leica recommendation. I'd love to hear from other people who
use this machine. Thanks in advance for your help!
Regards,
Erin Galati, HT (ASCP)
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ks do this?
Thank you!
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
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r
ely. Does anyone have a protocol
that might give the same results?
Thank you,
Erin Martin
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
Am J Dermatopathol. 1995 Aug;17(4):362-7.
Benzidine stain for the histochemical detection of hemoglobin in splinter
requirements and therefore
still want to see negative controls. Has anyone by inspected recently by TJC
and had this issue come up?
Thank you!
Erin Martin
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
Confidentiality Notice
The information transmitted is intended
Hello Histonet-
I was just curious as to what types of Deionized water systems you all use.
We are looking for an easy, low-volume system that creates Type II DI
water. Any information would be greatly appreciated. Thank you!
Regards,
Erin Sarricks, HT(ASCP
, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and
appeared to be a good fit for our needs. Does anyone have any other advice on
other machines we should look into? Any information or advice would be greatly
appreciated. Thank you!
Regards,
Erin Sarricks, HT (ASCP
No open flames.
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
Confidentiality Notice
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it is addressed and may contain confidential and/or priviledged material. Any
review
We use a 50% solution of Nair in water to soak nail blocks after we have faced
into them. Then albumin to keep them on the slides. Works great!
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
Confidentiality Notice
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embedded at 58C. Or am I missing
something? Does anyone have a clear cut answer to this?
Thanks everyone!
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
Confidentiality Notice
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Happy Monday!
We get ours from BioCare.
-Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
Confidentiality Notice
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it is addressed and may contain confidential and/or priviledged
nal immunopathologic diagnosis.
This is all foreign to me - we do IF on derm following an inherited protocol.
I've never worked up any IF. If anyone has thoughts on how to apply this to
skin I'd appreciate it!
Thanks
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
way to use HIER in waterbath or pressure cooker?
Thanks in advance,
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
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http://www.petfriendly.es/llahg/hxlke.wcdarfmxxhbo
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xylene in the cleaning cycle but it's only a gallon.
Good luck!
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
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it is addressed and may contain confide
dryness? Would you recommend soaking the block in ammonium hydroxide
or wiping the surface of the block with it between sections? Also,
what dilution would you use? Thanks in advance for your help!
Erin Sarricks, HT (ASCP)
Histology Team Leader
USAMRICD Comparative Pathology Branch
I agree
On Jul 18, 2012, at 2:35 PM, "Goins, Tresa" wrote:
> I vote No - this run would be valid only if you held all future slides
> overnight after HIER.
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu
out a good rapid derm
processing protocol on a conventional processor?
Thank you so much!
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
Confidentiality Notice
The information transmitted is intended only for the person or entity to which
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and found no
difference in special stains, immuno or H&E.
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
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Good morning everyone,
Does anyone use MCP antibody (Merkel Cell Polyoma Virus) from a vendor other
than Santa Cruz Biotech? If so, would you please let me know your source?
Thank you!
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
ttempts at modifying the retrieval process have not
been unsuccessful.
Has anyone else been running into these issues? Any advice or tips would
be greatly appreciated! Thank you.
Erin Sarricks, HT (ASCP)
Histology Team Leader
USAMRICD Comparative Pathology Branch
Office: Bldg E-3081 R
be greatly appreciated!
Thanks,
Erin
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would be
great! Thanks!
Erin Sarricks
Erin Sarricks, HT (ASCP)
Histology Team Leader
USAMRICD Comparative Pathology Branch
Office: Bldg E-3081 Room 178
E-mail: erin.p.sarri...@us.army.mil
Phone: 410-436-1967
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it to work like the BAP1 at
another facility who got it to work (intermittently only) on one of 2 Ventana
instruments.
Thanks in advance for your help!
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
rcuit boards... and it's still doing it. Has anyone
else seen this problem and if so how did they fix it?
Thanks in advance!
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
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to go that far.
Hope this helps!
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
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Try an extended prime when you start it in the am, it seemed to resolve the
issue with our glass coverslipper at least for now. (We were having a horrible
time with it, and that's what the repair man did. No bubbles for five days and
running!)
Erin G. Bertani, B.A.
Clinical Labor
Try an extended prime when you start it in the am, it seemed to resolve the
issue with our glass coverslipper at least for now. (We were having a horrible
time with it, and that's what the repair man did. No bubbles for five days and
running!)
Erin G. Bertani, B.A.
Clinical Labor
Hi Histonet-
Does anyone know of a good stain for mycoplasma? Our pathologist wanted to
try an overnight Giemsa stain, but I wanted to check and see if anyone knew
of another stain that might work well. Thanks!
Erin Sarricks, HT (ASCP)
Histology Laboratory Technician
USAMRICD Comparative
ank you in advance!
Thanks
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
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to stain, so
spitting on them is my last last last resort! Haha! Thanks in advance for
all your help!
Erin Sarricks, HT (ASCP)
Histology Laboratory Technician
USAMRICD Comparative Pathology Branch
Office: Bldg E-3081 Room 178
E-mail: erin.p.sarri...@us.army.mil
__
cal
regulatory agency, which would make it easier for them to give you guidance.
Erin Martin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
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. Does anyone have an idea of why
just that layer would overstain? On only a few slides of the set?
Thank you!
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
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Has anyone run into a problem or artifact from freezing spray? I think we may
be having a problem with it but I can't find any pictures or descriptions of
what it looks like.
Thanks in advance,
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353
7;s with Denise from Dako!
Erin
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
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your help!
Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248
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Hi,
We keep ours on by using egg albumin (from American Master Tech). Brush some
on the slide, let it air dry then pick up your sections. It will hold through
PASD as well as H&E.
Good luck!
Erin
PS A 1:4 Nair solution softens the block up nicely after you face it in.
Erin Ma
malin step on the processor? Enough to require some
retrieval but not as much as formalin fixed tissue? Has anyone else run into
this problem? If so, how did you solve it?
Thank you for you help!
Erin Martin
UCSF Dermatopathology
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Hi, Would anyone using SOX10 antibody please tell me where they buy it? If
you have a procedure that you would be willing to share as well I would be very
appreciative. Thank you!
Erin Martin
UCSF Dermatopathology
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We too were told by our internal safety compliance officer that 10% NBF has to
be stored in flammable cabinets and that is now how we store it. We were also
told that our general purpose household bleach has to be in a flammable
cabinet
Erin Martin
UCSF Dermatopathology
Hi everyone,
Are any of you using the Intellipath LIS? Not the Intellipath stainer from
Biocare. If so, I have some questions on how you use the procedures report to
get your orders.
Thanks!
Erin Martin
UCSF Dermatopathology
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Hi all,
>From the responses I have received, almost everyone loves their microwave
>processors for soft tissue biospies. Is anyone using a microwave on derm?
Erin
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Hi all,
What experiences have you all had with microwave processors? Sakura?
Milestone? We're trying to figure something our. I know there will be some
passionate responses...And I look forward to them!
Thanks so much,
Erin Marti
Hi Jeanine,
What are spacer coils? I don't think I've ever heard of them.
Thanks,
Erin Martin
UCSF Dermatopathology
From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:j...@cdc.gov]
Sent: Mon 12/22/2008 11:29 AM
To: Martin, Erin; histonet
S
they are divided between
different docs so the flats are would not follow numerical order... I know
it's expecting a lot to think that slides would be ready for file so fast. I
needed some other opinions to show them.
Thanks,
Erin
From: Michael Mihalik
convection oven for a few hours but the
slides still get all stuck together in the file. They will not consider film
coverslipping.
Does anyone else file this quickly? I am grateful for any suggestions!
Erin Martin UCSF Dermatopathology
Good morning,
Does anyone know of a source for antibody NGF p75 other than
Chemicon/Millipore? The last few lots we have gotten from them have been
dramatically weaker so I'd like to find a new vendor.
Thanks!
Erin
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