[Histonet] XS-3 xylene substitute processing times for GI tissue

Good afternoon, Are there any GI labs on here that use XS-3 xylene substitute? If so, would you mind sharing your processing times? Our lab is switching and I would like to try to get validations right the first time. Thank you, ​Jacque R. Sagasser, HT (ASCP)cm Gandhi GI Pathology, LLC 999 Bru

[Histonet] Xylene substitutes

Good morning My lab is thinking about switching to a xylene substitute to reduce the smell and toxicity being emitted from the lab. Could someone discuss the best alternatives to produce optimal results for colon and esophagus biopsies? What are the pros and cons of each? Thank you, ​Jacque R

[Histonet] Electronic requisitions

Hello Does anyone use electronic requisitions? If so, would you mind describing your system for staying compliant with CLIA? Is a paper requisition sent in addition to the electronic requisition? If not, how does that work with accessioning and matching the specimens and patient information? W

[Histonet] Controls

Good afternoon! Could any of you tell me off hand how old patient tissue has to be before you can use it as a control? My lab has only been operating since February. We run quite a few Alcian Blue PAS stains a week, and my last control is getting sparse. I have some beautiful patient tissue tha

[Histonet] p53

Hello, Our lab has been using this down time to validate a few antibodies. So far we have validated Ki67, AE 1/3, CK20, and CDX-2. I am in the process of validating the P53 (DO-7) antibody, but am having trouble producing positive expression. I am using the same colon adenocarcinoma tissue I us