Re: [Histonet] Freezing spray artifact

Erin - Try this trick when using the freezing spray: take a folded kim wipe tissue and fold it again, then again then the long fold is folded twice so you end up with roughly a 1 inch by 1 inch multi layer slide wiper. Run your finger down each crease and after the final fold, give it a little

Re: [Histonet] protein concentration for Cy2

Eric: Are you using Cy2 labeled goat anti-mouse IgG (H&L) from Jackson ImmunoResearch Labs? According to their catalog (product number 115-225-003), the stock is 2.0 mg/ml. At 1:100, your working concentration would be 20 ug/ml. Jim B "Breisch, Eric" Sent by: histonet-boun...@lists.utsout

RE: [Histonet] CD3 on mouse

Patsy - I agree. LV's rabbit MoAb works well on mouse tissue. They also have a rabbit polyclonal CD20 that works well on mouse. jb "pru...@ihctech.net" Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2010 11:58 AM Please respond to pru...@ihctech.net To "Mauger, Joanne" , "Emily

[Histonet] North Carolina Histology Meeting

To members and friends of the NCSHT: &nbs= p; On behalf of the North Carolina Society of Histopathology Technologist= s (NCSHT) we invite you to our 2010 NCSHT Spring Meeting, April 8 th <= /SUP>- 10 th in Research Triangle Park , NC . <= P class=MsoNormal

Re: [Histonet] Procedure for manual C4D immunofluorescence staining

Frozen sections cut at 3-4 microns. Air dry 30 minutes. Acetone fix for 5 minutes (room temp acetone), air dry slides PBS wash x2 C4d (diluted 1:500 in 1% BSA/PBS) incubated 1 hour. Mouse monoclonal C4d is from AbDirect/Serotec PBS rinse, wash x2 Cy2 labeled goat anti-mouse IgG (diluted 1:100 in 1

Re: [Histonet] DIF tissue in GLUT

Dear Anne, I assume the glutaraldehyde tissue has been processed and embedded in paraffin. Following removal of paraffin and any pretreatment to unmask the antigen, you can block autofluorescence or change the color by treating the sections with 0.05% sodium borohydride prepared in PBS for 30 m

[Histonet] Job Opening in North Carolina

The Immunopathology Laboratory at Duke University Medical Center in Durham NC has a technician/technologist position available. Please contact me for further information. Jim Burchette burch...@mc.duke.edu 919.681.3973 ___ Histonet mailing list Histone

Re: [Histonet] whipf's polychrome procedure

Here is the polychrome formula I use. Jim Polychrome Methylene Blue Stain Tissue and Fixation Frozen sections and formalin-fixed, paraffin embedded Objective Rapid differential staining of tissue sections. Metachromatic stain. Reagents Stock solutions: A. 12% aqueous potassium carbonate,

Re: [Histonet] Re: Knife strop photo?

I sent her a digital image of one we have here at our little hospital. Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" Robert Richmond Sent by: histonet-boun...@lists.utsouthwestern.edu 06/11/2009 01:40 PM To histonet@lists.utsouthwestern.ed

Re: [Histonet] immunohistochemistry

I second Mr. Troutman's recommendation using silane slides, = but air dry overnight. Once the slides have been baked and paraffin re= moved, place the slides in citrate buffer and heat them in a closed plastic coplin jar for 12 hours at 75 degrees C. Some epitopes can be

[Histonet] Re: [IHCRG] CMV antibody

Dear Jean, I am very fond of Dako's M0854 and find I can use it at 1:400-1:500 when pepsin is used as a pretreatment proteolytic enzyme as compared to HIER pretreatment with a citrate buffer. CMV is run everyday in my lab. Jim Burchette "Taylor, Jean" Sent by: ih...@googlegroups.com 05/20/

[Histonet] HTLV-1 IHC

Is anyone performing IHC on FFPE human tissue for HTLV-1? Many Thanks, Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] NC Society for Histopathology Technologists 2009 Meeting

The North Carolina Society for Histopathology Technologists invites you to our 2009 meeting to be held May first and second at the DoubleTree Biltmore Hotel in beautiful Asheville North Carolina. Call the DoubleTree Biltmore Hotel at 1-800-222-8733 for special room rates and reservations or vis

Re: [Histonet] NGF p75

Leica Microsystems # NCL-NGFR "Martin, Erin" <[EMAIL PROTECTED]> Sent by: [EMAIL PROTECTED] 11/12/2008 12:06 PM To histonet cc Subject [Histonet] NGF p75 Good morning, Does anyone know of a source for antibody NGF p75 other than Chemicon/Millipore? The last few lots we have gotten

Re: [Histonet] Pepsin

Gene: Dako and Biocare both sell the stable pepsin solution. It is easy to prepare if you want to make it yourself. I use this pepsin everyday. Here is the recipe that Dr. Brigati gave me. Best Regards, Jim Burchette Brigati’s 0.25% Stable Pepsin Solution Reagents Pepsin, #P7012, Sigm

Re: [Histonet] SV40 HRP protocol HELP

Dear Anne: I use the SV40 (catalog # DP02) from Oncogene, a division of Calbiochem. A dilution of 1:500 consistently works well from lot to lot. For manual or autostainer platforms, pretreatment is with Dako's Target Retrieval pH 6.1 solution for 20 minutes in a 100 C water bath. Following a