[Histonet] special coloration for fish gill chloride cells

2010-02-26 Thread Jamshid Amiri Moghaddam
Hello friends I have some gill tissues for chloride cells localization. Do you know a method for chloride cells coloration except immunohistochemical using NKA antibody??? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.ed

[Histonet] SEM for Spermatozoa

2010-02-16 Thread Jamshid Amiri Moghaddam
Hi every body We want to scan fish spermatozoa By SEM. But I don't know the method for dehydration after Post fixation with osmium tetra oxide? By ethanol series or aceton? I thanks to all who can help me in SEM for Spermatozoa. Regards: Jamshid -Original Message- From: histonet-boun...

[Histonet] RE: Liver Tissue

2010-02-10 Thread Jamshid Amiri Moghaddam
I have problem like Mike, my tissue samples were fixed one year ago in Boine and reserved in 70% ethanol. tissues are too hard. I decreased dehydration time and could take few good section, but after coloration, I couldn't take good photo from those. The sections (diameter: 4 micron) are absorbed t