Hello friends
I have some gill tissues for chloride cells localization.
Do you know a method for chloride cells coloration except
immunohistochemical using NKA antibody???
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.ed
Hi every body
We want to scan fish spermatozoa By SEM. But I don't know the method for
dehydration after Post fixation with osmium tetra oxide? By ethanol series
or aceton?
I thanks to all who can help me in SEM for Spermatozoa.
Regards: Jamshid
-Original Message-
From: histonet-boun...
I have problem like Mike, my tissue samples were fixed one year ago in Boine
and reserved in 70% ethanol. tissues are too hard. I decreased dehydration
time and could take few good section, but after coloration, I couldn't take
good photo from those. The sections (diameter: 4 micron) are absorbed t