Does anyone know of a commercial source for unconjugated anti-porcine
hemagglutinating encephalomyelitis virus that can be used on FFPE tissues?
Thanks in advance,
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary
How often are you decontaminating your NeXes machines (running
decontaminating reagents through the tubing)?
Jan Shivers
UMN VetDiagLab
- Original Message -
From:
To:
Sent: Thursday, May 20, 2010 2:41 PM
Subject: [Histonet] possible AFB contaminant
Please help, we have had an
Von Willebrand Factor (Factor VIII) is also what I use to stain pig
endothelium.
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
shive...@umn.edu
.
I've done species cross-reactivity studies with every antibody I have in
stock (>80), so if you send me a list of the antibodies you're interested in
(directly to me), I can let you know which ones cross-react with porcine
tissue, and which vendor works for me.
Jan Shivers
Senio
Smooth Muscle Actin; Dako; cat. # M0851, clone 1A4
Requires HIER antigen retrieval (I use a Biocare Decloaking Chamber with
Dako Target Retrieval Solution)
Strong staining on sheep tissue
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of
s you need from others.
Jan Shivers
UMN Vet Diag Lab
- Original Message -
From: "Burton, Lynn"
To:
Sent: Tuesday, February 02, 2010 12:50 PM
Subject: [Histonet] IHC protocol for vimentin in animal tissue
Does anyone out there have an IHC protocol, for Vimentin, in anima
2 BVD virus.
Thanks in advance,
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
shive...@umn.edu
(Confidentiality Notice: This message, together wit
had a
problem.
Jan Shivers
- Original Message -
From: "Nicole Collette"
To:
Sent: Friday, January 08, 2010 12:28 PM
Subject: [Histonet] shipping slides, elementary question
Happy Friday everyone,
I have a very basic question about shipping slides (mouse tissue,
no
Leica IP S Slide Printer
- Original Message -
From: "Igor Deyneko"
To:
Sent: Friday, December 18, 2009 10:44 AM
Subject: [Histonet] Slide Labelers
Dear Histonetters!
I was wondering if anyone out there is using a slide labeler. I would
appreciate any info on models(serial numbers),
of setting guidelines for my labs, but
want to be sure to be in agreement with other vet labs out there as well.
Thanks,
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
___
Histonet ma
http://www.ihcworld.com/_protocols/epitope_retrieval/citrate_buffer.htm
Jan Shivers
- Original Message -
From: "Justin Peters"
To:
Sent: Thursday, December 03, 2009 3:36 PM
Subject: [Histonet] buffer protocols
Can someone help me with a protocol for making citrate buff
I was told that they would not extend annual maintenance agreements to my
models older than 5 years, but they will come to service older models on a
repair or emergency call/fee-for-service basis.
Jan Shivers
- Original Message -
From: "McMahon, Loralee A"
To: "X
Does anyone know of a commercial source for anti-EMCV (porcine
encephalomyocarditis virus)? If so, please contact me personally at my email
address below.
Thanks in advance,
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary
anti-Rabbit 30' (with 4%
normal dog serum added per volume for blocking), AEC 10'.
Hope this helps,
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-
it for
your tissues. In my lab, it requires absolutely no tissue pretreatment, and
the stain is always strong, clear and clean. Good Luck!
Best regards,
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
answer? We're writing a sample storage SOP for each lab section today, and I'd
like to get an answer as soon as possible.
Thanks in advance,
Jan Shivers
UMN VDL
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwes
I'm posting this for a colleague
Does anyone have experience using glycerin/phenol as a storage medium, and if
so, for how long are the tissues still good for Histology processing/staining?
I assume they can't be used for IHC.
Thanks in advance,
Jan Shivers
University of
I'm posting this for a researcher. Does anyone out there do IHC testing for
Brucella canis on fixed tissue? If so, please contact me directly.
Thanks,
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Labor
knowledge) is important. This probably holds true for many
other institutions as well.
So, yes, get your BS degree. With it, you'll most likely go where you want
to go in your career, given time and perseverance. Feel free to contact me
personally, if you have any other questions.
Jan S
ing. Citrate buffer for antigen
retrieval also works fine.
Jan Shivers
Univ. of Minnesota
Veterinary Diagnostic Laboratory
St. Paul, MN
- Original Message -
From: "PALMER Jason (SVHM)"
To:
Sent: Tuesday, July 14, 2009 9:33 PM
Subject: [Histonet] RE: human vimentin IHC
r done oxytocin receptor IHC, so I don't know how difficult it is to
retrieve it on the best of days. Is there anyone out there who might know the
answer to this question?
Thanks in advance.
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Mi
Hello all,
I'm asking this question on behalf of a researcher here. Does anyone know of
the availability of an antibody that reacts with eosinophils, and if so, do you
know if it reacts with canine eosinophils?
Thank you in advance.
Jan Shivers
Senior Scientist
Pathology Teaching Pr
x27;t
put the brains face down on the ice tray. They are face up on the ice tray.
This way they don't soak up water, they just get chilled.
Jan Shivers
Section Head
HIstology/IHC/EM
Univ. of Minnesota Veterinary Diagnostic Lab
St. Paul, MN
- Original Message -
From: "Breeden, S
?
What temps to you perform IHC at?
How long are your incubations?
Etc.
Jan Shivers
UMN VDL
- Original Message -
From: "Perry, Samuel"
To:
Sent: Thursday, April 09, 2009 1:31 PM
Subject: [Histonet] Edge effect on IHC-P
Hi All,
I am having variable staining when performing IH
We mix up our own, using Tween 20 at 0.05% per volume. No problems here,
when using a Dako autostainer.
Jan Shivers
UMN Vet Diag Lab
- Original Message -
From: "Charles, Roger"
To:
Sent: Monday, March 30, 2009 9:53 AM
Subject: [Histonet] IHC automated buffers
Hello,
ime we use the
machine.
I must say, using this device has increased my positive staining perhaps
double of what I was getting with the microwave... and I thought I was
getting strong staining with the microwave! I've had to dilute out my
antibodies, which is a good thing.
Jan S
On FFPE tissue -
CD20
LabVision/NeoMarkers
cat. # RB-9013
Rabbit polyclonal
Does not need any tissue pretreatment
Works GREAT.
Jan Shivers
Univ. of Minnesota
Vet Diag Lab
- Original Message -
From: "Edwards, R.E."
To:
Sent: Monday, March 30, 2009 6:22 AM
Subject: [Histon
gen by using the wrong AR method. However, it does require
you to multitask, if your lab has a heavy workload and you have to perform
AR outside of the autostainer. Most days I'm running HIER with 3 different
types of retrieval solutions, as well as doing enzyme digestion at the same
time on
r via
email when an SOP is up for reviewal, if you have a system of annual
reviewals in your institution. This is a very handy reminder tool.
Jan Shivers
Section Head
IHC/Histo/EM
UMN Vet Diag Lab
St. Paul, MN
- Original Message -
From: "Laurie Colbert"
To:
Sent: Monday, Ma
4 um here.
Jan Shivers
UMN VDL
- Original Message -
From: "Vanessa J. Phelan"
To:
Sent: Tuesday, March 03, 2009 8:14 AM
Subject: [Histonet] Section thickness
Hi Guys,
Just wondering what thickness you cut sections at? I was always used to
cutting at 2-3 microns in m
IDEXX - they bought the Ab rights to the clone previously produced and
sold
by Cornell. But it's very hard to get them to sell it to you. They use
it to create their own ELISA test kits, I believe.
Jan Shivers
UMN VDL
- Original Message -
From: "Breeden, Sara&qu
Short answer to part of your question - (veterinary) paraffin blocks and
stained slides stored for 7 years. I'll ask our necropsy personnel how long
they store additional wet tissues.
Jan Shivers
UMN Vet Diag Lab
- Original Message -
From: "Jeanie Meyers"
To
Can anyone direct me to a good photo of an IHC-stained Borrelia burgdorferi (+)
tissue?
Thanks in advance,
Jan Shivers
UMN VDL
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Please tell us what chromogen stains and counterstains you are using, and
the type of mounting medium for the coverslip. This will help us try to
answer your question.
Jan Shivers
UMN VDL
- Original Message -
From: "shazana hilda"
To:
Sent: Monday, February 02, 20
records, etc. in this program.
Jan Shivers
Histology/Immunohistochemistry/EM Section Head
UMN Veterinary Diagnostic Laboratory
- Original Message -
From: "Sorenson, Jon (Nampa)"
To: "Histo Net"
Sent: Thursday, January 29, 2009 2:19 PM
Subject: [Histonet] Document C
stainer is level.
6) As others have mentioned, make sure that your slides are completely
dewaxed.
Jan Shivers
UMN VDL
- Original Message -
From: "Paula Lucas"
To:
Sent: Wednesday, January 21, 2009 6:04 PM
Subject: [Histonet] Auto-IHC Staining Issues
Hello,
I've
face.
Jan Shivers
UMN Vet Diag Lab
- Original Message -
From: "Steven Coakley"
To:
Sent: Wednesday, January 14, 2009 2:53 PM
Subject: [Histonet] Work flow, quality issues
I have worked in several HT labs and as expected most differ in individual
technique. Most are very g
wear
nitrile gloves and disposable lab coats and booties, and stain the slides on
dedicated autostainers.
As others have said, check out the CDC website for more information.
Jan Shivers
UMN Vet Diag Lab
- Original Message -
From: "Joe Hardin"
To:
Sent: Thursday, Januar
ystems, etc.)
So... what do you charge for tissue/tumor antigen IHC? Infectious disease
antigen IHC? I'm not looking for a long list of prices just an average fee
per slide.
Thanks in advance for any help you're able to provide.
Jan Shivers
Senior Scientist
Pathology Teaching Pr
min in AEC. I use
an autostainer, but it can be done manually with the same incubation times
at RT.
This works for me on many mammalian species.
Jan Shivers
UMN VetDiagLab
St. Paul, MN
- Original Message -
From: "Merced Leiker" <[EMAIL PROTECTED]>
To:
Sent: Wednesday,
Has anyone ever done IHC on parafomaldehyde-fixed tissues, and if so, how well
did it work? Will the same antigen-retrieval methods used with formalin-fixed
tissue be applicable?
I'm asking for an investigator, who already has his tissues fixed in
paraformaldehyde.
Jan Shivers
S
HIER on the CD3
slides... though the high pH will most likely remove some of your cells.
If you use alcohol or acetone as your fixative, you won't need to do any
HIER.
Jan Shivers
- Original Message -
From: "Woodward, Denise" <[EMAIL PROTECTED]>
To: "Sa
Me, too!
Jan Shivers
- Original Message -
From: "Pat Flannery" <[EMAIL PROTECTED]>
To:
Sent: Tuesday, November 18, 2008 1:21 PM
Subject: [Histonet] Re: Check out this MSDS exerpt for HistoClear
Paula-
Where can I get one of these?
-Pat Flannery
Reminds me of
iciency testing, equipment calibration data, and all of our SOPs.
You can find out if this is what you're looking for by going to their
website (entering in 'Q-Pulse' in your search terms is more direct).
Jan Shivers
- Original Message -
From: "Maray Weirauch" <[EMA
Joe,
You might try going to a listserv for Electron Microscopy. You can find it
at:
http://www.microscopy.com
Jan Shivers
- Original Message -
From: "Joe" <[EMAIL PROTECTED]>
To:
Sent: Friday, November 14, 2008 11:21 AM
Subject: [Histonet] old electron micros
7;t need that length of time at
high pressure/temp. I have an extra Decloaker and am considering switching to
this device for my normal case work HIER, if it's time efficient.
Please reply privately. Thanks.
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teach
Rabbit anti-CD20, B cell (Lab Vision/NeoMarkers; cat. #: RB-9013-P;
Immunogen: synthetic peptide derived from C-terminus of human CD20 protein)
This antibody has worked on every mammalian species on which I've tried it.
Does not need ANY tissue pretreatment! Stains brilliantly.
Has anyone ever fixed and serial sectioned whole (turkey) eggs, including
shell? I'm looking for a lab that might be able to accommodate such things.
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St.
We instituted an online system called QPulse. It tracks procedure SOPs,
equipment calibration, training records, etc. You might check that out.
Jan Shivers
IHC/Histo/EM Section Head
UMN Veterinary Diagnostic Lab
St. Paul, MN
- Original Message -
From: "kristen arvidson&quo
ly rapidly. Thus,
the reason why microwaving works best for me. Others will have different
needs and time allowances in their labs.
P.S. a) I always start with cold buffer prior to heat retrieval. b) I
always cooldown in HIER buffers for 20'.
Jan Shivers
UMN VDL
- Original Me
have experience with
their products, and if so, which model works well for you in performing antigen
retrieval, and is the instrument calibratable? Please respond to me
personally. Thanks.
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagn
Does anyone know of a good source for a Neospora caninum antibody, other than
VMRD?
Thanks in advance.
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
[EMAIL PROTECTED
could use to block binding to platelets (or what
appears to be platelets, though it could be bits of proteinaceous cellular
debris that's binding Ab nonspecifically)?
Jan S
- Original Message -----
From: Jan Shivers
To: histonet
Sent: Tuesday, October 07, 2008 3:14 PM
Subject: platele
Hi all,
Has anyone ever encountered platelets staining nonspecifically when using an
antibody directed against other antigens? If so, how do you quench that
activity? Thanks.
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
101 - 154 of 154 matches
Mail list logo
