We have pretty much given up on finding certified techs willing to relocate (we
are in central California). We have mostly turned to trainees from within our
lab. We have actually found some very good techs that way. And then there are
always the other local labs that are willing to pay more
Hi Donna- I have been dealing with these same exact issues for 3 1/2 years now
so I think I have achieved "expert status" in dealing with this problem.
First, I'll give you my current protocol. When all things are working
smoothly, it produces a nice stain. But it is very frustrating when it
Hi Alison- many years ago I worked in a hospital. We had a very small Frozen
Section room in the OR. We did keep a 5 gallon carboy of formalin in there for
the convenience of the OR staff so they would have formalin available for their
specimens. We were told by someone in "authority" (sorry
Subscribe, please. Jeff Robinson, Sierra Pathology Lab, Clovis, CA.
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Hi Estela- I had the same problem with the Bond multicytokeratin. I currently
have 3 vials of the "old" one on backorder. I had to validate the new
"reformulation" as I have now run out of the old one (PA0909). Have your rep
send you the new one (PA0012). My rep sent me one for free. It se
Good Morning- can anyone give me a recommendation for a predilute PAX2
antibody? The one I was using has been discontinued and the replacement
product does not seem to perform very well. I will be using it on the Bond
III. Thanks in advance!
Jeff Robinson, Senior Histotechnologist, Sierra Pa
An additional word of caution about placing the EM Lab under ANY kind of
potential water leak. Tim Morken and I can attest to that! They installed a
new CT scanner in Radiology directly above the EM Lab. They broke some sort of
water pipe during the installation and it leaked so much that par
Hi Karen- I have also had problems getting tissue being stained for IHC
markers to adhere to the slide. The Potassium permanganate is really harse on
the tissue (if you can get the sections to stay on). Here is a trick I picked
up at an NSH lecture that I have used successfully several times.
Hi Melissa- when Tim Morken taught me to be an EM tech many moons ago we would
take a small dissecting microscope to the CT room. We would have the
radiologist place the first core on a sterile tongue depressor and then we
would check it in some saline on a dental wax square under the scope.
Hi Edison- I have had some headaches getting the SOX-10 to run in the past but
I now have all of the kinks ironed out and it works quite well. I had started
with the CellMarque mouse monoclonal of their RTU SOX-10. I had it working
well and then Leica recalled their red detection kits. I cou
Good Morning to all: I am having staining problems with my PAX-5 predilute
from CellMarque. I was wondering if anyone else has experienced a dramatic
dropoff in staining signal with this antibody after a few months. I have now
had this happen to me twice in the last 3 months. It stains very
Good Morning and Happy Holidays to all my fellow Histotechs- We are having
problems with our Ventana iScan digital imaging system. It breaks down
frequently and is causing extreme frustration for my pathologists. It has
gotten so bad we are trying to revive the old Ventana VIAS and use it ins
Hi Richard- I have been using the CellMarque IgG4 (MRQ-44 clone), mouse
monoclonal on the Leica Bond with success.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.
-Original Message-
From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Good Morning to all Histotechs- There have been many questions over the past
several months regarding poor retic staining on the Ventana Benchmark Special
Stainer. With the help of my histotech friends, I have now achieved successful
staining on both liver and bone marrow specimens utilizing t
We run IHC overnight on Friday night and IHC for the Saturday pathologist (on
call pathologist on a rotating basis with 17 total pathologists). We have
several pathologists who use Saturday as a catch up day and will come in to
read their pending IHC stains.
Jeff Robinson, Sierra Pathology Lab
I would love to have some talented staff with degrees! I have a staff of
around 22 people but I am currently the only HTL (I have a BA in Biology). I
do not have anyone else who is even eligible to sit for the HTL exam. It would
be very nice to have someone to share the load with!
A friend of
Hi Brian- we just buy controls for spirochetes. I did procure a control block
many years ago from someone working with pigs in a vet histology facility in
Missouri. It was quite good as I recall. That may be an option for you. We
run so few Steiners that it is not worthwhile for me to try a
Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I
don't recall using the Ventana H. pylori but I was using the CellMarque H.
pylori for years. It would often have a lot of background as you describe. I
run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The sli
We used an alternative tape (I believe it was from Mercedes) years ago and we
had some major issues with the tape detaching from the slide over time (in
storage) and taking the tissue on the slide with it. Perhaps the xylene being
dispensed needed to be adjusted or some other technical problem
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