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Joe Nocito, BS, PACM, HTCM (ASCP) QIHC
Dept of Pathology/ 59LSQ/SGVLH
Lackland AFB, TX 78236
joseph.noc...@us.af.mil
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Joe Nocito, BS, PACM, HTCM (ASCP) QIHC
Dept of Pathology/ 59LSQ/SGVLH
Lackland AFB, TX 78236
joseph.noc...@us.af.mil
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I was working with a dermpath, who's friend is a dermatologist setting up
their lab. They stopped dead in their tracks. I mean 88305s would be their
bread and butter.
Joe
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
It might be the antibody itself. We are seeing the same problem. My doctor
also thinks that our antibody is picking up normal flora also. Now, when I
QC the slides I have to go by morphology. We have a couple of reps coming by
to boast about their HP. Let you know how that works out.
Joe
-Ori
Lila,
Please drop me an email. I have some information for you. Thanks
Joe Nocito, BS, PACM, HTCM (ASCP) QIHC
Dept of Pathology/ 59LSQ/SGVLH
Lackland AFB, TX 78236
joseph.noc...@us.af.mil
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Histonet
else doing out there? We are using the Ventana UltraView
detection kits. Everyone who uses these kits know how expensive they are.
Is 5 and 5 sufficient or should go by CAP recommendations?
Joe Nocito
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet
When I was in Texas the second time ( I was there from 1981-1986 and
returned in 1991. OK so I'm dating myself) , we were preparing a CAP
inspection. I found a brown bottle way in the back of the chemical cabinet.
Lo and behold it was the picric acid I saved from the previous go round. I
said to m
My question is "are they being found with empty Lonestar beer cans. Inside
joke. Years ago in Texas, Lonestar Beer was running commercials with
armadillos running across the street carrying Lonestar Beer. See, down here
in Texas, armadillos are usually found as road kill. The commercials had
them b
When I was at the AFIP and I had desk duty, I was afraid to walk through
that 5th floor at night where all the animals were. I always wondered what
they were doing up there. Now I know, adaptable armadillos with leprosy and
ever increasing intelligence scouring D.C. I wonder if they are trained to
turn you to regular programing.
Joe Nocito
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William
Chappell
Sent: Thursday, May 24, 2012 6:03 PM
To: Davide Costanzo
Cc: histonet
Subject: Re: [Histonet] Unregi
yeah, I never did like that the "a monkey can do it" crap. A pathologist
told me that he could teach a monkey to gross. When the grossing tech messed
up a case, I was called in and got jumped on. He really didn't appreciate it
when I told him he better get that monkey trained quick.
JTT
I would appreciate that Tony
- Original Message -
From: "Tony Henwood (SCHN)"
To: "'Joe Nocito'" ; "joelle weaver"
; ;
; ; "Histonet"
Sent: Thursday, January 26, 2012 3:39 PM
Subject: RE: [Histonet] Interview Questions
Joe,
I
I used to give a 10 question test on general histology. I also had the
expected answers written down and on my copy. Was accused once of being a
racist. What saved me was having the answers in front of me. The person
didn't get one answer correct. I had a couple of embedding questions, some
cut
I start with the holidays. We rotate the major ones every year. When I set
up the private lab I used to work at, I had it put in the H.R. policy that
an employee could take 2 of 3 end of year holidays (Thanksgiving, Christmas,
New Year's) on a rotating basis. If no one else put in for those time
I wonder if it works with Scotch. Hmmm, seems like an experiment brewing.
JTT
- Original Message -
From: "Tony Henwood (SCHN)"
To: "'Della Speranza, Vinnie'" ;
Sent: Monday, December 19, 2011 4:04 PM
Subject: RE: [Histonet] Has anyone else noticed
I keep trying to verify this scie
Bob,
try Mopec. When I was at this year's NSH in Cinci, they had a few different
sizes and configurations.
Joe the Toe
- Original Message -
From: "Bob Richmond"
To: "Histonet@lists.utsouthwestern.edu"
Sent: Thursday, December 08, 2011 2:19 PM
Subject: [Histonet] pathology grossing s
Can't agree more. I mean, where would Joe the Toe be without the Histonet?
By the way, just read the reviews from the lecture my buddy Hector and I
gave in Cinci this year. One of the critiques stated that Hector and I are
the biggest egomaniacs this side of space. Wow, I've been called a lot of
My son is heavily in high school activities now. I have to drive him to all
these school events. I know I'll probably pay for this later down the road,
but I'm actually pushing him to get his driver's permit so he can drive. by
time we get home, it's time for my beauty sleep. If you saw me in Ci
I'm here. I was asking my Priest the steps to perform an exorcism, although
the Catholic Church doesn't perform these rituals any longer. I have all the
materials, readings and "exorcism paraphernalia" Just let me know where and
when. Just make sure there is a good bottle of Scotch available, j
I was stationed at AFIP from 1986-91. I was in the Immuno lab. I thought I
was doing "imunos" when I was stationed in San Antonio, TX (remember the
Dako kits?) I wasn't even close to the things that AFIP was doing. We were
making our own rabbit antibodies. Made up our own link and label from
s
Greetings Histoland,
in a pinch, we've also used mayonnaise. We opened one of the small packets
you get with sandwiches and smeared a little of it onto a slide and there
you go. Also, if you can obtain a fresh pierce of kidney with Renal Cell
carcinoma, that will work also.
Joe
- Original
Greetings all,
I contacted CAP just before my on-site inspection last year. I was told that
all validation studies had to be on file until the antibody/ special stain/
or equipment was no longer used. Sad to say. Now, I understand for 2012, CAP
has added another "lab specific" checklist. My und
would that be the Red Sox or the White Sox. Sorry, couldn't resist. Had a
very bad day
Joe
- Original Message -
From: "Thomas Jasper"
To:
Sent: Wednesday, July 27, 2011 11:01 AM
Subject: [Histonet] Sox 10
General question -
Is anyone out there running Sox 10 on the Ventana Bench
Greetings and Salutations Histoland,
I have a question about denatured alcohol. I work in a government facility
and absolute alcohol (200 proof) is still considered a controlled substance.
This requires a monthly inventory by someone from another department. Years
ago (ok many, many years a
yes. I'm in the beginning stages of validating a new immuno machine. Because
we have over 120 antibodies, I'm guessing about 2500 slides. Guess who pays
for that testing? Certainly not CAP. I hate monopolies. Even though I know
labs who dropped CAP and went with JCAHO, CAP is still a monopoly. L
I wouldn't decolorize the slides. Just remove the coverslip and hydrate to
water. Any antigen retrieval should take out the Eosin. You're going to
counterstain in Hematoxylin any way. Years ago (ok, many moons ago) I did an
informal study. I took 10 antibodies, stained two sets of controls H & E
Just wanted to thank you all for your advice, likes, dislikes and experiences.
I am really honored to be part of this group. Seriously. It's refreshing.
Joe
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Greetings all,
if you had to purchase new tissue processors, which one would you choose?
Microwave technology is out of the question. Are Sakura's still a good buy?
We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help
Joe
___
I would also see if Ventana can send you some test slides to test the
heating. At my old lab, we had a problem with the heating plates. They
repaired the entire platform. Just a thought.
Joe
- Original Message -
From: "Mark Tarango"
To: "Dessoye, Michael J"
Cc:
Sent: Wednesday, Feb
#x27;Liz Chlipala'" ; "Weems, Joyce" ;
"Joe Nocito" ; "Histonet"
Sent: Tuesday, February 08, 2011 5:37 PM
Subject: RE: [Histonet] IHC validation
When changing instruments you are validating the instrument, not the test.
For each antibody you just need to ru
I was devastated to learn that IHC is not considered High Complexity
Testing. I agree with Bonnie that we do not report results, therefore, IHC
is not considered high complexity testing. Sad but true. Somebody pass the
Scotch. I'm way past Tylenol
JTT
- Original Message -
From: "Whita
Greetings Histoland,
I need some help. We are about to switch IHC machines from the Richard-Allen
Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run
for the validation study? We have over 100 primary antibodies. Normally, when
we work up a new antibody, we start wit
Greetings all,
I'm going to show my ignorance (like that hasn't happened before). When
accessing the archives, how do I do it? Do I search by topic or date? All the
time I've been on here, I never had to use the archives. Thanks. Oh Oh, I feel
another tear coming
Joe
___
Just wanted to thank all of you for your responses. I may be a little
sentimental and biased, but this is still a great forum. You all are great. I
belong to another list server (which will left unnamed) and that "other" place
doesn't even come close to the Histonet. Crap, I feel a tear or two c
Greetings histo land,
Hope everyone is having a great New Year. I have been asked to consult on IHC
to a local firm. They asked me what my fees are. I told them we can talk about
that later. I have no idea what to charge these people. I want to be fair, but
I don't want to give this knowledge aw
thanks y'all for the info
Joe
- Original Message -
From: "Feher, Stephen"
To: "Joe Nocito" ; "Histonet"
Sent: Monday, December 06, 2010 4:15 PM
Subject: RE: [Histonet] Hemoglobin A
We just got some from BioGenex order number is AR021-5R.
Ste
Howdy histoland,
does anyone know where to get Hemoglobin A? Dako doesn't have it any more and
we've tried Biocare, Cell Marque, ThermoFisherLabVisions etc, etc, etc,
Biogenex. No luck. Thanks
Joe
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open season. Which reminds me, those
of you who live in a tourist town, how come when it's tourist season, you can't
shoot them? There's deer season, bird season, crab season and tourist season.
Just a thought.
- Original Message -
From: "Mike Pence"
To:
speaking of,
I guess my last posting rustled some feathers. I received a phone call from
my rep saying that upper management wants to talk to me. I told him that
there wasn't anything to talk about. Except that I was remiss. I want to
thank Chris for all her work in the lab. I'm sorry that I di
we've had them for over two years and we still don't have the system up and
running (for various reasons, some internal issues, some external issues).
We had to stop automatically sending the slide queue from the cassette queue
because of a lot of confusion with extra slides. A lot of slides wer
yeah, I'd be careful of this method. We used to do this at the U.S. Air
Force hospital I used to work at when I was active duty. One time, the fire
alarm went off and we had to evacuate the lab. Well, there must have been a
real problem because we were out of the lab a long time. By the time we
Greetings histoland,
does any one know of a company that sells adenovirus controls slides? I've
tried NewComer's Supply, MasterTech, Biocare, Biogenex, ThermoFisherRichard
Allen, or whatever they're called this week. Thanks
Joe
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Happy Friday all,
try 10% sodium hydroxide for 1 hour prior to processing. It is important to
let the slides drain really well before placing them in the oven. You want
to make sure there is no water between the section and the slide. We use
Plus slides with plain distilled water in our water b
I see that now, wasn't the first time I was wrong today, probably wouldn't
be the last either
- Original Message -
From: "Jeffrey Silverman"
To:
Sent: Wednesday, March 31, 2010 5:03 PM
Subject: [Histonet] Just three months?
CAP is now saying no more gross processing of small th
I've seen lab get accredited one month, only to have CLIA come through the
next month and rip that lab apart. I have been through CAP inspections that
consisted of the inspector looking only at slides while he checked off the
checklist to inspectors carrying their own thermometers and testing th
just had a lively discussion at work. My take is that the only thing CAP
changed was that they combined the "processing" and "grossing" pieces
together again, which I don't know why they split them in the first place.
But you don't have the entire CAP note and many people miss this. The last
i
depends. A fine Scotch whiskey may be aged in old oak casks, while a
delicate Tequila may not be
- Original Message -
From: "Breeden, Sara"
To: "histonet"
Sent: Tuesday, March 02, 2010 10:44 AM
Subject: [Histonet] Alcohol Source?
I hate to even ADMIT this and I ought to use a disguis
Once upon a time in a far away land, we used to boil our embedding molds in
boiling soapy water, over an open Bunsen burner, followed by an alcohol
rinse then air dry. One time the fire alarm was activated and we had to
evacuate the hospital. We were out there quit awhile. When we received the
Thermo Fisher or whatever they are called this week has it
Joe
- Original Message -
From: "Rene J Buesa"
To: ; " Ruth A (NIH/NIDCR) [E]Yaskovich"
Sent: Friday, February 26, 2010 2:28 PM
Subject: Re: [Histonet] Crystal/Mount
Why you just don't Google it?
René J.
--- On Fri, 2/26/10
Pam is correct, I mean, look at me, I've been denatured for many years.
Happy New Year everyone
JTT
- Original Message -
From: "Pamela Marcum"
To: "Smith Wanda"
Cc:
Sent: Monday, January 04, 2010 9:52 AM
Subject: Re: [Histonet] Problems with Denatured Alcohol?
Wanda,
The first
20% sodium hydroxide works great. Hey, they don't call me "Joe the Toe" for
nothing. Happy New Year
JTT
- Original Message -
From: "DELIA GARCIA"
To:
Sent: Thursday, December 31, 2009 1:26 PM
Subject: [Histonet] Nail softner
Hi Everyone,
I have a question... What is a good nail so
we use 20% sodium hydroxide. Works great
- Original Message -
From: "Cheryl"
To:
Sent: Wednesday, December 16, 2009 4:08 PM
Subject: [Histonet] looking for KOH nail procedure
Hi Guys!
I've used a potassium hydroxide solution to 'soften' nails after fixation
but before processing. I
I have a question similar to this one.
If I take 2 IVD antibodies say cytokeratin AE1/AE3 and 34BF12 and make these
into one cytokeratin cocktail, is this considered an ASR because I combined
them into another antibody?
Joe
- Original Message -
From: "Morken, Tim"
To: "Foshey, Annet
Debbie,
as far as I know, there is no modifier, all you can charge is a 88307.
That's the same case when you have a skin excision at a 88305. Even if it's
a melanoma excision and it takes 20 blocks, you can only charge an 88305.
The system is FAR from perfect.
JTT
- Original Message -
Oscar? when I was the supervisor of the immuno lab at the AFIP, our rabbit
that produced our anti-keratin died. The civilian researcher and I were
working up multiple cytokeratin cocktails to replace Fluffy. We wanted to
call it the BOZO antibody, but our medical director refused. I can see it
We soak our nails in 20% sodium hydroxide for at least an hour, rinse in
running tap water for a few minutes before we place the cassettes on the
tissue processor. We use positive charged slides, heat slides in 80 degree
oven for 20 minutes and perform a PAS/fungus on the slides. The PAS is a l
I wouldn't say that histology is a dead end job, I mean, I jumped to a PA
and I know that I couldn't have passed that exam without my histology
experience. The idea of adding an HTL category was initially a good one.
Trying to bring us up to the MT and CT levels, but honestly, I think it fell
s
let's stop monkeying around OK? (couldn't resist)
JTT
- Original Message -
From: "Maria Katleba"
To: "LINDA MARGRAF" ;
Sent: Monday, July 13, 2009 1:48 PM
Subject: RE: [Histonet] misleading message on Histonet last Friday
OMG... I do not like primates (especially gorillas)... So
descriptions. I
wish I knew about this book before I took my PA exam.
Joe Nocito, BS, PA, HT (ASCP)QIHC
San Antonio, TX
- Original Message -
From: "Karin Groeger"
To: "Jaime Plata" ;
Sent: Friday, June 12, 2009 3:10 PM
Subject: RE: [Histonet] Grossing techniques
Greetings all,
I'm going to admit that I'm a dummy and need help with getting info from the
archives. I'm specifically looking for postings dealing with using freeze spray
while doing frozens. So, how do I get to the archives again? That sucking noise
is me pulling my head out of my butt. Thanks
Schaundra,
we have residents and 2 PAs perform all the grossing. In real tight
situations, we have a couple of qualified grossing histotechs we can rely on
in a pinch. we have histotechs set up the specimens, but the residents and
PA's gross alone. We are responsible for annotating any special
this is hard to do because of the frozen section requirement. However, we
make smears out of mayonnaise when we have Oil Red O stain and it works
well. Good luck
JTT
- Original Message -
From: "Steven Joy"
To:
Sent: Thursday, April 23, 2009 1:38 PM
Subject: [Histonet] commercial con
at are named with the accession number. The printouts with sections
mapped
are kept in a binder in the cutting room where a pathologist can find them
when the need arises.
Sate
On Fri, Apr 17, 2009 at 6:30 PM, Joe Nocito wrote:
like Mike,
we only photograph unusual specimens. Seems photog
like Mike,
we only photograph unusual specimens. Seems photographing specimens has
become less and less important. Kind of like autopsies.
JTT
- Original Message -
From: "Mike Pence"
To: "Steven Joy" ;
Sent: Friday, April 17, 2009 4:20 PM
Subject: RE: [Histonet] Gross Photography
I second that. Marsha is the loveliest person I know.
- Original Message -
From: "Denise Piontek"
To:
Sent: Wednesday, April 08, 2009 8:49 PM
Subject: RE: [Histonet] Controls needed!
Newcomer Supply has been a reliable control source, when necessary the have
"customized orders".
we're running one negative normal serum slide per block. For example, if
we're performing mouse antibodies i.e. CD-45, HMB-45, pan Cytokeratin, we
run one IgG mouse normal serum control. If we are running rabbit polyclonals
(or rabbit monoclonals) and mouse monoclonals on the same case, we usual
another issue could be the mixer blowing too hard. I've had that happen too.
JTT
- Original Message -
From: "Kari Bradshaw"
To: "Dana Spears" ; ;
"Phyllis Thaxton"
Sent: Wednesday, April 08, 2009 2:13 PM
Subject: RE: [Histonet] Benchmark XT
We just recently went through a similar p
how about having my name: Giuseppe, Pasquale, Enzo Guido Nocito. I think I
was named after a pizza. That's why I call myself Joe. (The toes came later,
heh,heh,heh)
JTT
- Original Message -
From: "Bernie Taupin"
To: "Va Paula Sicurello" ; "Jeanine
(CDC/CCID/NCZVED)Bartlett" ; "R.E.Ed
yeah, what she said
- Original Message -
From: "Bernice Frederick"
To: "'Bernie Taupin'" ; "'Bryan Llewellyn'"
; "'Histonet'"
Sent: Wednesday, April 01, 2009 3:10 PM
Subject: RE: [Histonet] Off topic posts
How would we survive without those witticisms of JTT f we disallowed off
top
OUCH
JTT
- Original Message -
From:
To: "Jennifer Anderson"
Cc: "Histonet"
Sent: Thursday, March 26, 2009 9:50 PM
Subject: Re: [Histonet] Please help! In dire need of user manuals
Dear Jennifer and any other Histonetters that are of like mind,
It's not quite "Flaming Friday" yet,
here we go and it's almost Friday. I received a call just like that at my
last job. Me being me, I gave her a piece of my mind ( not too much, I don't
have that much to go around). Told her she had some big testicles (not the
word I used, but you get the idea) and hung up. I then called in the t
can you say "GRINCH"?
- Original Message -
From: "Ingles Claire " <[EMAIL PROTECTED]>
To: "Merced Leiker" <[EMAIL PROTECTED]>
Cc:
Sent: Thursday, December 04, 2008 2:08 PM
Subject: RE: [Histonet] JCAHO
Yesterday was the closest I have ever come to going 'Histo'. After 2 pretty
much
lunch!?
- Original Message -
From: "Edwards, R.E." <[EMAIL PROTECTED]>
To: "'Bernice Frederick'" <[EMAIL PROTECTED]>; "'Joe Nocito'"
<[EMAIL PROTECTED]>; "'Histonet Alias'" <[EMAIL PROTECTED]>
I thought that was Purple Haze, by Jimi Hendrix. Oh dear me, is it Friday
yet? Forgive me, we are in the window for CAP and I'm just all in a haze.
JTT
- Original Message -
From: "Histonet Alias" <[EMAIL PROTECTED]>
To: "Marshall, Kimberly" <[EMAIL PROTECTED]>
Cc:
Sent: Wednesday, Dec
have you tried Lab Storages Systems, New Comer's Supply or Surgipath?
JTT
- Original Message -
From: "Pamela Marcum" <[EMAIL PROTECTED]>
To:
Sent: Tuesday, December 02, 2008 3:40 PM
Subject: [Histonet] Large Slide Boxes
Good Afternoon All,
I am looking to purchase some 3 inch by
if I
remember correctly. In this era of financial uncertainties, foreclosures and
all, I don't blame Al. I'm sure he/she has bills just the rest of us do.
Bills that won't go away if he/she loses the job.
That's my story and I'm sticking to it.
Joe Nocito BS, PA, HT(
is a casual histo tech like a casual end table? What is it the rest of the
time?
Oh, yeah, we not only have a great cents of humor, we're real cut ups to.
JTT
- Original Message -
From: "Dianne Holmes" <[EMAIL PROTECTED]>
To: "Histonet"
Sent: Tuesday, December 02, 2008 10:44 AM
Subj
e
only reason to hide your identity (fear of Ventana sending the hounds after
you).
On Fri, Nov 28, 2008 at 3:19 PM, Joe Nocito wrote:
> no, they usually contact the CEO's of the labs to get their employees to
> stop ragging on the company. Then again, you never k
no, they usually contact the CEO's of the labs to get their employees to
stop ragging on the company. Then again, you never know who is lurking in
the winds.
JTT
- Original Message -
From: "Mark Tarango" <[EMAIL PROTECTED]>
To: "Histonet Alias" <[EMAIL PROTECTED]>
Cc: ; "Martin Trotte
oh yeah, many times. I had three at my last place. One was always going down
for one reason or another. I wanted them to replace the machine, but they
kept putting bandages on a bleeding wound. I went up to the regional
maintenance manager and never got an answer.
I guess the Lemon Law doesn't a
it might be your processor. Depending on the type of processor, some
processors have a rotary valve that rotates from reagent bottle to reagent
bottle. When the rotary valves fails, it starts placing a reagent in the
reagents bottles indiscriminately. For instance, our processor started
spitti
Jessica,
in my experience, CAP trumps JCAHO. Every hospital that I've worked in that
the lab was accredited by CAP, JCAHO left the lab alone, except for taking a
walk through and maybe asking some safety questions.
JTT
- Original Message -
From: "Vacca Jessica" <[EMAIL PROTECTED]>
To
great. Soon there will be only one booth at the states and the NSH
conventions
- Original Message -
From: "Bernice Frederick" <[EMAIL PROTECTED]>
To: "'Jack Cates'" <[EMAIL PROTECTED]>;
Sent: Wednesday, November 12, 2008 2:46 PM
Subject: RE: [Histonet] RA Lamb has been sold to the e
blood pressure medicine, geez, I want some of that Gold Seal alcohol. And I
don't want to unsubscribe (did I spell that right?)
JTT
- Original Message -
From: "Victor Tobias" <[EMAIL PROTECTED]>
To: "Ford Royer" <[EMAIL PROTECTED]>
Cc:
Sent: Thursday, November 06, 2008 4:25 PM
Subject
I'm allergic to blue cheese.
- Original Message -
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <[EMAIL PROTECTED]>
To: "Joe Nocito" <[EMAIL PROTECTED]>; "Caroline Bass" <[EMAIL PROTECTED]>;
Sent: Thursday, November 06, 2008 4:41 A
200 proof is 200 proof, can't get any purer than that. Of course, I would
take the taste test first. Green olives stuffed with Feta cheese goes good.
JTT
- Original Message -
From: "Caroline Bass" <[EMAIL PROTECTED]>
To:
Sent: Wednesday, November 05, 2008 11:15 AM
Subject: [Histonet]
it's a good thing you asked.
I let my nails (well not my nails personally, but the specimen nails) soak
in 20% ammonium hydroxide or 20% sodium hydroxide for at least 1 hour
(sodium hydroxide works better, but whatever you can get). Then I rinse the
blocks in running water for 2-3 minutes, then
87 matches
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