Re: [Histonet] IHC and Histogel

2018-05-10 Thread John Shelley via Histonet
I agree with Dusko, I have used histogel the same way and have also used it for FISH and ISH without any issues. Kind Regards!   John J Shelley Histology Core Manager Sanford Burnham Prebys Medical Discovery Institute at Lake Nona 6400 Sanger Road    Orlando, FL 3282

[Histonet] Florida Society for Histotechnology

2018-05-01 Thread John Shelley via Histonet
Hi All, If you are interested in getting some CEU's, we have a great line-up of workshops. Click on link below for program details: http://www.fshgroup.org/wp-content/uploads/2018/03/FSH_Program_May_17_20_2018-rev.pdf The meeting will be taking place at the Renaissance Tampa International Plaza

[Histonet] 2018 FSH Annual Meeting

2017-11-14 Thread John Shelley via Histonet
Hello Histonetters! The FSH Annual Meeting will be in Tampa, FL on May 17-20, 2018 at the Renaissance Tampa International Plaza Hotel. We are looking for ideas for classes that you will be interested in attending. We are also looking for speakers. Send abstracts to email below. We want to hear

[Histonet] alpha-glycerophosphate dehydrogenase staining protocol

2017-06-21 Thread John Shelley via Histonet
Hi All, I have a group that wants to do an enzyme stain called alpha-glycerophosphate dehydrogenase on mouse skeletal muscle and was wondering if anyone has a working protocol. Thanks!!! Kind Regards! John J Shelley ___ Histonet mailing list Histone

[Histonet] Frog tissue

2017-02-21 Thread John Shelley via Histonet
Good Morning Histonetters, My daughter's class dissected frogs and her teacher gave me a frog to dissect and make histological slides and blocks for her. I have taken a bunch of tissue and not too sure how well preserved the tissue may be but I thought I would give it a try. I am sure that some

[Histonet] Plant tissue

2016-10-21 Thread John Shelley via Histonet
Happy Friday! I have given a request for service to me to do some plant tissue histology and I must admit this is a new area for me. I plan to do both paraffin and frozen sectioning and would like to ask if there may be anyone has a protocol on how to handle such tissue in regards to a tissue p

[Histonet] Florida Society for Histotechnology Spring Meeting

2016-03-07 Thread John Shelley via Histonet
Hi All, Happy Monday! I would like to inform all histonetters both in Florida and abroad that our meeting program has been finalized and registration is now open. We have a wonderful array of workshops that will appeal to all levels of experience. We have our 3rd annual All-Day Supervisor and

[Histonet] Tissue Arrays

2016-01-27 Thread John Shelley via Histonet
Good Morning, I have purchased some arrays from a company in the past and had some very varying results. I prefer not to mention the company and so my request is if those of you who do not have the luxury of having tissue at your disposal to make your own tissue arrays from whom are you buying

[Histonet] 2016 FSH Annual Meeting Abstract Request

2015-10-01 Thread John Shelley via Histonet
Good Morning, I know that there are many people out there that have a passion to teach what they know and we would very much like to put a call out to those who would be interested in presenting a workshop or two at our 2016 Florida Society for Histotechnology Annual meeting in the beautiful gu