[Histonet] Politics in the Lab ; Turn around times vs Scientific sanity

2016-02-28 Thread John Smallwood via Histonet
s fixation; Goldstein NS, Hewitt SM, Taylor CR, Yaziji H, Hicks DG. Recom- mendation for improved standardization of immunohistochemist- ry. Appl Immunohistochemistry Mol Morphol 2007; 15:124-33. John Smallwood, MLT. STEGH Gen. Hosp. St.Thomas,

[Histonet] Routine lab Formalin Fixation

2016-02-27 Thread John Smallwood via Histonet
What are people using for minimum fixation times in NBF 10% for tissues in a routine mixed sample load Histology Lab, on a routine VIP 5000 processor I have concerns1.) for Bio-safety,2.) Immunohistochemistry, 3.) Routine samples nuclear staining (no alcohol f

[Histonet] Celestin Blue B

2015-01-06 Thread John Smallwood
Hello Histologists: Is there anywhere to get this ? Celestin Blue B C.I. 51050 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Basement Lab

2014-05-03 Thread John Smallwood
Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? Than you, John Smallwood, MLT. London, Ont

[Histonet] Leitz 1512

2012-05-27 Thread John Smallwood
Does anyone have a resource for parts for this 40 year old microtome ?? Thanks John Smallwood ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Outdating- Embedding Wax and Mounting Media

2011-05-14 Thread John Smallwood
To all Quality Control Purists There seems to be a growing concern with regards to outdating on reagents and their continued use beyond this date. I notice that Histological wax and Mounting media are dated with an expiry date. Just begging the question-should we go back. melt down and re-em