[Histonet] remove my email addres

2012-01-18 Thread Kant, H.J.G. van de (Henk)
Dear Histonet, Although I truly appreciate all the members for helping me in the past, please remove my email address from the list. Thank you. Henk van de Kant ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern

[Histonet] Tlr 9 immuno

2011-10-04 Thread Kant, H.J.G. van de (Henk)
Hi All, Can somebody help me with ordering a TLR 9 antibody, which is working on mouse tissue, formalin fixed and paraffin embedded. Including the procedure and the name of the company. Greetings, Henk van de Kant Utrecht Institute of Pharmaceutical Sciences Faculty of Sciences, Utrecht Uni

[Histonet] Toluidine blue

2011-09-27 Thread Kant, H.J.G. van de (Henk)
Hi All, Have someone a good workong protocol for staining mastcells in cytospin slides with Toluidine Blue. Mention also the pH and fixation. Thanks, Henk van de Kant Utrecht University, Faculty of Science, Division of Pharmacology, David de Wied Building, Universiteitsweg 99, 3584 CG, Utrecht

[Histonet] instructions for steaming slides

2011-08-24 Thread Kant, H.J.G. van de (Henk)
Dear All, Can somebody help me with instructions (protocol) for steaming slides in a domestic ricecooker or vegetable steamer. Head induced antigen retrieval method is with sodium citrate 0.01 M pH 6.0. Kind regards, Henk van de Kant | Utrecht University | Faculty of Science | Department o

[Histonet] Apoptosis staining on mouse testis

2011-08-18 Thread Kant, H.J.G. van de (Henk)
Here a protocol for Bouin fixed tissue and it is working on testis. TUNEL analysis on Bouin fixed, paraffin embedded tissue * Incubation box in stove (37°C) with wet tissues. * Dewax. All steps 3 min 3x Xylene, 2x Ehanol 100%, 1x Ethanol 96%, 1x Ethanol 70% and 1x MilliQ water. * Mi

[Histonet] T-bet antibody

2011-08-17 Thread Kant, H.J.G. van de (Henk)
Dear Colleagues, Has somebody results with an anti mouse T-bet antibody, who is working fine on paraffin sections and formaline fixed. Best Regards, Henk van de Kant ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsout

[Histonet] cd4 from angio proteomie

2011-08-15 Thread Kant, H.J.G. van de (Henk)
Hello, Has somebody used the CD4 antibody from Angio proteomie. It is an anti mouse and it is for paraffin IHC. Catalog # 2H9 Is it working? Henk van de Kant Utrecht Institute of Pharmaceutical Sciences Faculty of Sciences, Utrecht University visiting address: Universiteitsweg 99, room 2.21