[Histonet] (no subject)

I'm all set to DYI my own phosphomolybdic/phosphotungstic acid solution for running a Masson Trichrome, but I see that the former reagent was purchased as "phosphomolybdic acid hydrate". All the recipes I have seen call for just "phosphomolybdic acid" but that is not a reagent that appears to exis

Re: [Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background)

I suspect the "all red" as opposed to "only some red" is because the PSRed is not de-staining out of the non-collagen structures. My guess is that your Solution B is old. Acetic acid becomes weak in water very fast so I make the 1% acetic acid solution fresh every time and use the same day. Hope

[Histonet] Re-embed agar/FFPE tissue in plastic?

I have a bunch of precious FFPE samples that were embedded in agar prior to being embedded in wax (so as to orient the tissue easily). The client now wants these samples to be taken backwards out of wax and processed for semithin plastic GMA (JB-4) sectioning. Is that possible? Will the tissue ha

[Histonet] Faded Masson trichrome

This is quite a puzzle. I ran a handful of slides in the SAME manual run for my Masson trichrome stain (one glass caddy of slides, passing through single troughs of dyes). I've just coverslipped them and half the slides have the expected bright high contrast coloring that I usually see, and half

[Histonet] Leica microtome, buying used vs. new?

Hi All, I need to acquire a Leica RM2235 microtome. I've got a quote from Leica directly, and several quotes from refurbished / used ones too with warranties for 6 months parts and labor. So here's my question: *does anyone recommend strongly against getting refurbished models?* The list seems