[Histonet] Correction

2024-07-15 Thread Ken M via Histonet
In my recent post I meant HSV1&2 tissue blocks not HIV sorry. Kevin Cooper Tissue Procurement Specialist kdea...@hotmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Post

2024-07-15 Thread Ken M via Histonet
Please do not throw away your expired tissue blocks. Some of them can be very valuable for research and clinical diagnosis. If your organization's policy allows you to sell them, we will pay a fair price. If not, we can provide your lab with valuable consumables, and we will provide a contract t

[Histonet] HSV1/2, CMV, Adenovirus

2024-06-25 Thread Ken M via Histonet
Hi: Anyone who may have HSV1/2, CMV, Treponoma, Adenovirus tissue blocks we would be happy to hear from you. We can trade almost any other rare block you can think of or even buy them if your lab allows this policy. Our research partners need them. Kevin kdea...@hotmail.com ___

[Histonet] Control Slides

2024-02-24 Thread Ken M via Histonet
May I ask, in all your opinions, Who is supplying the best control slides for clinical settings? Both Special Stain and IHC? What would you like to see in your control slides? What don't you want to see- long lead times, quality? As for Research, what are your requirements? _

Re: [Histonet] Tissue falling off slides

2024-01-31 Thread Ken M via Histonet
1) Try tap water 2) Try hydrophilic IHC slides, (I will send you samples for free) Ken kdea...@hotmail.com -Original Message- From: richard cartun via Histonet Sent: Wednesday, January 31, 2024 10:29 AM To: histonet@lists.utsouthwestern.edu; Foshey, Annette Subject: Re: [Histonet] Tiss

[Histonet] Aspergillus

2024-01-19 Thread Ken M via Histonet
Can anyone provide me with Aspergillus tissue embedded blocks? Prefer good quality naturally occurring but will accept good quality home grown. We usually use Placenta, but the tissue doesn't matter much. Will buy or trade. kdea...@hotmail.com ___ Hist

[Histonet] IHC/Special staining

2023-09-13 Thread Ken M via Histonet
Does anyone know of any lab with excess capacity good quality and fast turnaround that could do IHC/special staining on the side? We are out of capacity, and we don't always have the antibodies that we need. Kevin kdea...@hotmail.com ___ Histonet maili

[Histonet] MOHS staining

2023-06-12 Thread Ken M via Histonet
For those doing MOHS staining either manually or automatically. How many staining cups do you really use? Are six enough if there is a running water cup nearby or do you need more? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://

[Histonet] What they don't want you to know.

2023-05-18 Thread Ken M via Histonet
How can you make some of your Histology equipment last forever? Water bath- fill the water every morning with water at about the same temperature that you operate. Every one of these water bath units contain a heating element that is the biggest source of failure. The less you make it work mean

[Histonet] Sections sticking to tweezers

2023-05-06 Thread Ken M via Histonet
Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separati

[Histonet] Looking for laboratory assistant

2023-04-20 Thread Ken M via Histonet
High volume, fast growing non-clinical laboratory in Durham, NC looking for a laboratory assistant. Some experience staining, cutting on Microtome and printing slides a plus, but not necessary. Willingness to learn to use the Biocare Oncore Pro IHC staining platform a plus. Work closely with lab

[Histonet] SV40

2023-04-19 Thread Ken M via Histonet
Does anyone have a tissue block of IHC SV40? We can swap with you for almost every other block you can think of, or buy it from you. We have a partner desperate for it to complete a study. Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.ed

Re: [Histonet] Blade holder assitance

2023-04-07 Thread Ken M via Histonet
Charles: Is there a spacer under the blade to convert hi to lo profile? Is there a spot for one? You may be missing, or have the wrong spacer. Ken From: Charles Riley via Histonet Sent: Friday, April 7, 2023 3:34 PM To: histonet@lists.utsouthwestern.edu Subje

[Histonet] Problems with Melan A staining

2023-03-15 Thread Ken M via Histonet
One of my histotech friends is having issues with Melan A staining. Using MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue control was stained strongly positive while the positive melanoma tissue control was stained weakly positive. He couldn’t figure out why after severa

Re: [Histonet] Expired Tissue Blocks

2023-01-12 Thread Ken M via Histonet
Whitaker Ohio State University AP Operations Director 614-293-8418 -Original Message- From: Ken M via Histonet Sent: Thursday, January 12, 2023 3:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Expired Tissue Blocks External Email: CAUTION Don't throw your expired emb

[Histonet] Tissue blocks

2023-01-12 Thread Ken M via Histonet
If that were true, all cancer research and antibody development in the USA would come to a complete halt. Get a signature from someone who died of cancer 10 years ago?? Here are the findings of most US courts. Here are the facts. In legal disputes concerning the research use of excised tissues,

[Histonet] Expired Tissue Blocks

2023-01-12 Thread Ken M via Histonet
Don't throw your expired embedded blocks in the trash. Some of them can be very valuable for research and antibody development. If you can provide a brief description of any positive block (no patient identifiers or names please), we will pay you top dollar to take them and send you all shipping

[Histonet] Tech Needed

2023-01-10 Thread Ken M via Histonet
We are looking for a part-time technician for our fast growing Durham, NC lab. A retired Histotech would be ideal. No live patients or grossing. Mostly cutting and staining processed and embedded blocks. Some IHC experience would be great, but not necessary. Contact Helena at hmur...@springside

[Histonet] AFB blocks

2020-08-25 Thread Ken M via Histonet
Does anyone know where I can obtain good Acid Fast Bacteria tissue blocks? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Acid Fast

2020-08-11 Thread Ken M via Histonet
Hi everyone- We have used a modified Ziehl Neelson stain on some breast tissue and found round shape organisms that are Acid Fast and not rod-like. Is anyone familiar with this before I send them to our Pathologist? Ken ___ Histonet mailing list Histon

[Histonet] Apple Green Birefringence in Amyliod slides

2020-07-28 Thread Ken M via Histonet
Hi everyone. I was wondering if anyone out there has any experience with diagnosing Amyloid tissue using Congo Red stained Kidney using polarized lenses. Is it common to use polarized light to detect Amyloid deposits? Does the absence of the "apple green birefringence" indicate a problem with

[Histonet] Gram Staining

2020-06-30 Thread Ken M via Histonet
Hi. We are trying to stain a supposed Gram Positive paraffin Tonsil tissue block using Gentian Violet, Iodine (Lugol), Gram's decolorizer, Carbol Fuchsin Counter and Tartrazine and are having trouble distinguishing the positive from the negative. Anyone have any suggestions? _

[Histonet] Anyone having difficulty with tissue adhesion?

2020-06-16 Thread Ken M via Histonet
Hi everyone We have developed a new hydrophilic IHC microscope slide that uses a new adhesive process that dries up to 50% faster than a normal Silane coated charged slide and we are looking for someone that might be using a Ventana or who may be dealing with difficult tissue or a difficult pro

[Histonet] H&E Staining Protocol

2020-05-28 Thread Ken M via Histonet
Hello: Does anyone have a good manual or automatic H&E staining protocol that will work well on multiple Paraffin embedded tissues? Ken kdea...@hotmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/ma

[Histonet] Recommended thickness of Amyloid sections

2020-04-16 Thread Ken M via Histonet
Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsout

[Histonet] Question concerning H-Pylori Staining

2020-04-08 Thread Ken M via Histonet
Hi everyone! We have a question about staining for H-Pylori Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,Toluidine Blue stock, Sodium Hydroxide) we notice what clearly looks like the H-Pylori purple stained clusters, but after dehydration in 100% alcohol the purple

[Histonet] Paraffin Tissue Blocks

2020-03-24 Thread Ken M via Histonet
To anyone interested: Please don't throw your old tissue blocks in the trash after they age out after 10 years. Many of them can be used by researchers and Pathologists and are badly needed. If you don't want to sort them out, we will pay you a fair price for the whole lot as long as we have a

[Histonet] Tissue Blocks

2020-03-16 Thread Ken M via Histonet
Hello All: I have thousands of tissue blocks from a large US certified reference lab. Most of them were used for IHC diagnosis. I am looking to trade what I have for more common Histology blocks for control slides such as Mast Cell, Pneumocystis, Copper, Gram, Aspergillus fungus, H-Pylori, Spir