Did you change paraffins lately? Softer paraffins, ie., lower melting point,
have more compressibility.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
- Original Message -
From: "Va Paula Sicurello"
To: "HistoNet"
Sent: Wednesday, March 11, 2009 11:29 AM
Subject:
What type of mucin? What type of cell? What is it's origin?
PAS will stain neutral mucin.
Mucicarmine works better on epithelial acid mucins, rather than connective
tissue acid mucins. But it will stain both sulfated and carboxylated acid
mucins.
Alcian blue (pH 2.5) and colloidal iron work
Sulfonate Alcian Blue (SAB) is not always as specific as you would like.
Also, if you are using the newer alcian blue dye, it seems to have a
different formulation than the old stuff that I have. The new alcian blue
will not dissolve in alcohol, so the newer dye doesn't demonstrate the
amyloid,
