[Histonet] Used Laboratory Equipment

2011-04-11 Thread Leslie Chen
Hi, Does anyone which are better websites to place ads to sell laboratory equipment that are reliable and well-trafficked? We are looking to sell a Tecan machine. Thank you! Leslie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lis

[Histonet] Re: Permount question

2009-09-10 Thread Leslie Chen
Thanks for all the replies, I guess these weird Asian technicians who are highly looked up to taught me something wrong and it's been propagated in the department. Thanks again! On Thu, Sep 10, 2009 at 1:03 PM, Leslie Chen wrote: > Hello, > Did anyone ever use Permount straight with

[Histonet] Permount question

2009-09-10 Thread Leslie Chen
Hello, Did anyone ever use Permount straight without cutting it with some solvent? Journal articles mention using Permount to coverslip, and the post-doc I work with thinks that it isn't diluted. I think maybe people just universally "know" that it should be diluted so it isn't mentioned in the Ma

[Histonet] detergent

2009-03-11 Thread Leslie Chen
Hi Rene, To clarify your post: Do you mean liquid dish soap (like palmolive) is to be used? When you say not dishwasher detergent I think of Automatic Dishwasher detergent such as Cascade. Or no dish soap at all and I should use liquid laundry detergent? Thanks. Leslie From: Rene J Buesa >

[Histonet] cryostat adapter

2009-02-02 Thread Leslie Chen
Hi everyone, I am reposting because it seems my photo of the embedding rings was throwing everyone off. I am looking for an adapter to fit in our Leica CM 1850. Not the rings themselves. The adapter would look like this, specifically made for the rings. http://www.myneurolab.com/myneurolab/mnl_p

[Histonet] cryostat adapter

2009-01-30 Thread Leslie Chen
Hello, I have been trying to find a cryostat adapter for 6 months with no luck. Does anyone know where I can find an adapter to hold plastic embedding rings such as this: http://www.emsdiasum.com/microscopy/products/histology/imaages2/62350__.jpg We have a Leica and I've called Leica, called Therm

[Histonet] 200 proof

2008-11-06 Thread Leslie Chen
Our MBG Ethanol is made by Gold Shield Chemical Co in Hayward, CA. It's called Rossville Gold Shield Ethyl Alcohol Leslie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Protect fingers during Microtomy

2008-10-28 Thread Leslie Chen
They make cut resistant gloves for kitchen staff in restaurants to keep workers from cutting their hands. Don't know if this would work for microtomy as I think it would get in the way of sectioning. . Leslie IMPORTANT WARNING: This email (and any attachment

[Histonet] gluteraldehyde and IHC

2008-10-17 Thread Leslie Chen
Does anyone know if the gluteraldehyde would also obliterate immunoflorescence staining as well? Does gluteraldehyde always affect IHC staining? I have some LacZ stained embryos that have been paraffin processed and I was going to try to optimize flourescence staining on them. Thanks. Leslie

[Histonet] histology ink washing off

2008-10-13 Thread Leslie Chen
Thank you everyone for your help. It seems the consensus is to use acetic acid as a mordant which worked. I have tried just blotting the tissue, but it never seems to completely "dry". The tissue has been fixed in gluteraldehyde and stained with the LacZ protocol and then post-fixed with PFA.

[Histonet] histology ink washing off

2008-10-10 Thread Leslie Chen
Hello, I am having a problem with histology tissue marking ink washing off specimens in water, PFA, or 70% alcohol. This is before the tissue even gets processed for paraffin embedding. Does anyone have any suggestions on how to keep the ink from coming off? Thank you. Leslie ___