Good afternoon- Has anyone run into problems when trying to perform IHC using rabbit mono or polyclonal antibodies on pig tissues? I typically do not have any issues with nonspecific staining or high background when using mouse monoclonal antibodies. I have used a variety of blockers including various sera in the diluents and blocking reagents. All samples are FFPE. I routinely use the DAKO EnVision+ Dual HRP system. Thanks in advance!
Lori Sorrells Research Associate II Revivicor, Inc. 1700 Kraft Drive, Suite 2400 Blacksburg, VA 24060 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet