[Histonet] Paraffin histology with Muscle

Dear all, Does anyone have optimization tips for using paraffin histology with muscle? We do have access to a cryostat to do frozen histology, but it's not our lab's thus we have to pay to use it. We do have all the materials necessary for paraffin-based histology though. I've noticed that muscle

[Histonet] H + E Staining protocols

Dear All, There are many variants to the standard H + E protocol. I'm seeking to optimize the current protocol our laboratory uses (pasted below). Do you guys have any suggestions of optimization (e.g. cutting down on the long water rinse after hematoxylin and increasing bluing reagent time)? Or i

[Histonet] Eliminating air bubbles when mounting coverslip

Dear all, Does anyone have good advice on preventing or eliminating bubbles when coverslipping? Thanks! Lucas Olson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet