RE: [Histonet] Leaving sample over night in ETOH 70% during fixation

Storing your samples overnight in 70% ethanol after fixation won't harm your samples, but they should be adequately fixed prior to this. I do not like xylene for mouse tissues, it makes them too brittle. Instead I used histoclear 11 from National Diagnostics. Histoclear 11 is less expensive than

[Histonet] a Perfect Red

Dear All, Thank you all for your contributions to my request for information about "A Perfect Red", Yes I should have searched it on the Net. Just didn't think! Anyway I shall be ordering it and will enjoy reading it. Thank you all a million. Margaret Miss Margaret Bl

RE: [Histonet] MSDS info

. Regards Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -Original Message- From: histonet-boun...@lists.utsouthwester

RE: [Histonet] off topic/on topic

Hi Andi, Would you kindly let me have the reference for "A perfect red", please? Thanks Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 0122

[Histonet] Thank you

Hello everyone! Thank you all for your good wishes on my retirement. I knew you were all great people: you have just proved it big time! Thank you all from the bottom of my heart. Keep smiling! Much love Margaret ___ Histonet mailing

[Histonet] This is goodbye

;ll get to meet some of you by chance during my travels. Best wishes to you all and a great big Thank You, From Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills

RE: [Histonet] No patient ID: Ink dissolved from Cassettes duringprocessing.

Pencil does not dissolve in processing reagents and is safe. I have also found the marker pens from Surgipath to be the best of those I have tried, but with the proviso that the labels should be left to dry for a couple of hours before being immersed in ethanol. I routinely use one of these pens

RE: [Histonet] [Help] oil red O stain in fattty change liver

without it. I have been given livers treated both ways. Plus slides: Superfrost plus, available from all good histology suppliers; ours come from VWR. Good luck. Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke&

RE: [Histonet] science for kids

Me too, even in the UK! Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -Original Message- From: histonet

RE: [Histonet] RE: OT: April Fool's Nonsense

I like that one! But I have to say I would have been feeling very, very squeamish had I been a fly on the wall. Sally here's to a happy retirement for you. If you come over to the UK, look me up in Cambridge. Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories

RE: [Histonet] Stupid Rabbit primaries!

from Menarini Diagnostics, website http://www.dako.com/uk/ar38/p107410/prod_products.htm. I found these useful for rabbit polyclonals. Good luck Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hosp

[Histonet] Fat tissue

provide more support? Regards Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/3

RE: [Histonet] Formalin-fixed paraffin embedded question

from the Society for histotechnology. I found this invaluable and now most of my tissues can be sectioned without soaking at all. I just chill them slightly, if necessary. Good luck with your samples. Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4

RE: [Histonet] FFPE tissue - sucrose fixing necessary?

This sounds like something you would do before freezing not processing to paraffin. Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/3

RE: [Histonet] fixation question for IHC

lute it in your preferred buffer. I have to say that this was about 10 years ago! Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -O

[Histonet] Beta Galactosidase antibody

Hi Everyone, Does anyone have a favourite antibody for beta Galactosidase that they would be confident to recommend? Thanks a lot. Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke&#

RE: [Histonet] Animal tissue processing

day as long as it is started early enough; if not, a delay must be set in order that the samples are not left in hot wax for extended times - refer to instrument manual. Good luck Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Me

RE: [Histonet] RE: Static issues

just move the blade along or replace it and it often cures the problem. Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -Original Message