Re: [Histonet] ZInc formalin

fin embedded mouse tissues. The morphology of the > section will be suboptimal due to this novel fixative. > If anybody has any experince with this Zinc Fixative please would you share? > > Regards, > > Mesruh Turkekul > mskcc.org >

[Histonet] teeth sectioning

Dear Histonetters, I have one more question. Is it possible to obtain 5-10um thick sections of PMMA embedded teeth using regular Leica paraffin microtome (RM2265) equipped with disposable tungsten carbide blade? Thanks, Mes On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekul wrote: > D

[Histonet] Re: Histonet Digest, Vol 112, Issue 1

Dear Histonetters, I am studying bone and teeth growth in rat maxilla. I will inject calcein green and would like to fix, embed and sections the rat maxilla. Any suggestions for the best method to fix, embed and section the samples for fluorescnet microscopy? Thank you very much! Mes HTL (ASCP)

[Histonet] Bluing

Dear Histonetters, I wonder what is the purpose of bluing the hematoxylin. Can bluing effect the clarity of the nuclear staining? Can one achieve different bluing results varying the composition, pH or time of the bluing reagent? Mes ___ Histonet mail

[Histonet] elmer' s glue slide coating protocol

Dear Histo Wisdom, I would like to use Elmer' s glue (I have Elmer's glue Glue-All multi-purpose glue) to coat slides so that my FFPE mouse femur sections remain on the slide after HIER during IHC. Is one type of Elmer' s glue better than other types? Do you have a working protocol? Please help m

[Histonet] bacteria staining

I would like to stain FFPE mouse intestine sections to demonstrate most of the bacteria in the normal flora of the intestine. Any suggestions for the simplest histological stain that will show most of the bacteria? Thanks, Happy holidays! Mesru mskcc.org __

[Histonet] Coverslipper

I use Leica cover slipper it is great, never gives problem Mesruh Sent from my iPad On Nov 25, 2011, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wid

[Histonet] thick paraffin sections

low melting paraffin going to help? Thanks, Happy holidays! Mesruh Turkekul mskcc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Leica coverslipper and Ventana slides

I use histoclear in the last step of dehydration and have no problem with the arm picking up the slides. If I leave the slides for more than one hour in the last histoclear and if the histoclear covers the labels, then the glue of the label dissolves and interferes with the arm picking up the slide

[Histonet] fixation, tissue processing for paraffin embedding and sectiong of the mouse eye

Anyone has a good protocol to fix, process, embed and section mouse eye? Prefered fixative is formalin. Any suggestions are welcome Thank you! Mesru Turkekul mskcc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwes

[Histonet] 10% NBF vs. 3.7-4% formaldehyde

Concentrated formaldehyde solution (37-40%) can be diluted in water, buffer, alcohol etc. to prepare different fixatives. One example is 10% NBF. When concentrated formaldehyde is diluted 1:10 in buffer (usually sodium phosphate) with close to neutral pH and some preservatives and stabilizers ad

[Histonet] Background in FFPE glandular tissue sections during IHC

kind of background? Do you know any treatment to block mucins or connective tissue fibers from nonspecifically and electrostatically attracting IgGs and detection reagents? Regards, Mesruh Turkekul mskcc.org ___ Histonet mailing list Histonet

[Histonet] Azure B for melanin

peroxide or K permanganate/oxalic acid treatment. Any comments will be greatly appreciated! Regards, Mesruh Turkekul mskcc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] TUNEL on Ventana

Hi Guys, Has anyone of you done TUNEL on Ventana? Mesruh Turkekul mskcc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Frozen mouse embryo

the blocks at -80C. Whenever I thaw frozen tissue and freeze it again the morphology is horrible. And with embryos I would not recommend it. Please email me if you need stage specific fixation times. For embryos bigger than E12.5 fix them overnight at room temperature. Best, Mesruh Turkekul

[Histonet] Congo Red control slides

Hi, I would like to know if anybody is purchasing positive control tissue slides for congo red staining. Can you suggest any vendor and catalog number? Thanks, Mesru Turkekul mskcc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://l

[Histonet] aqueous mounting media

are using. Alternatively you may use Mowiol 4-88 from Calbiochem. Mowoil does not contain any free radical scavenger chemicals and prevents photobleaching, it compatible with almost all fluorescent dyes. Best, Mesruh Turkekul MSc, HTL(ASCP)QIHC turke...@mskcc.org

[Histonet] Sterilizing cassettes

You can use sterilization without heat for plastic cassettes like ethylene oxide or UV. Most of the hospitals have ethylene oxide sterilizers. Or leave the empty labeled cassettes in formalin overnight and they will be sterilized. Mesruh Turkekul MSc, MLT, HTL(ASCP)QIHC turke...@mskcc.org

[Histonet] Cell blocks

How do you prepare your cell blocks? Mesruh Turkekul MSc, MLT, HTL(ASCP)QIHC turke...@mskcc.org Sent from my iPad fern ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] thick sections and thaw freeze OCT tissues

should be the thicker you cut the more difficult it will be. Mesruh Turkekul turke...@mskcc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Histonet Digest, Vol 93, Issue 14

We usually fix 16-20hrs in NBF, then do 5M EDTA pH=8.0 for 5-10 days at RT until the bone has soften. Place enough EDTA and change EDTA once in a while (like every other day) to avoid saturation of EDTA with Ca. After decalcificaiton is completed I re-fix the bone is NBF for another 6-8 hours. Then

[Histonet] IHC edge effect

correct. Regards, Mesruh Turkekul Memorial Sloan-Kettering Cancer Center New York, NY 10021 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Histonet Digest, Vol 73, Issue 33

Hi Naira, The RBCs have a heme groups that have very high autofluorescence. They will glow with every filter in every color. They will glow even without any staining. Just try to image unstained section and you will see. "*Autofluorescence* is the fluorescence