[Histonet] Suggestions for alternative fluorescent nuclei stains (that are not DAPI) ?

Hello all, I perform immunohistochemistry (IHC) and immunofluorescence (IF) on fixed free-floating rat brain sections (~50um thickness). Currently I use Prolong Gold with DAPI as a mounting media as well as a stain for the nuclei. I am seeking an alternative (fluorescent) nuclei stain for f

[Histonet] Achieving Better Antibody Penetrance in my Tissue Sections (IHC)

Hi All, I'm new to graduate school and the world of immunohistochemistry. I am looking for a way to change my protocols so that the tissue sections are stained/penetrated by the antibodies more effectively. I am doing an anti-substance P stain, followed by an anti-RFP stain in 50um sections