[Histonet] Trypsin question

Dear Members, Recently we're having some antigen retrieval difficulties with our ihc protocols. My question is; what is 1:250 or 1:300 trypsin (is this dilution or purity rate?) and are these sutiable for antigen retrieval? Which brand or kind of trypsin do you recommend? __

[Histonet] caspase 3 ihc on rabbit tissue

Dear Friends, We are planning to perform active caspase 3 immunohistochemistry on FFPE rabbit cornea tissues. Most antibodies I have found are not working on rabbit. Any suggestion??? Thanks in advance... Dr. Necat Yilmaz Mersin University TURKEY __ ESET NOD32 Antivirus Akıllı Güv

[Histonet] novocastra eNOS antibody on rat tissues???

Dear All, We're planning to study FFPE rat aorta specimens with anti endothelial nitric oxide synthase (NOS-3) antibody. We want to perform that with Novocastra brand anti-eNOS antibody (Product Code: NCL-NOS-3). According to datasheet the antibody works on human tissues only, other species no

[Histonet] mercury vs led lights

Dear Colleagues, We're attempted to buy a new inverted fluorescence microscope for our research lab. Distrubitor of leica offered us a new type system working with led lights which is about same price. Does anybody know advantages and disadvantages of this system compared to conventional (with

[Histonet] donation request

Dear Colleagues, We are expecting a quite busy period in terms of research studies. As some of you do also experiencing similiar issues, we are using fluorescent microscopes from other depts when available. But mostly it is not easy. Buying a new one does not seem possible soon due to financial

[Histonet] Antibody for ZP Proteins

Dear Colleagues, We're planning to study ZP1, ZP2 and ZP3 protein immunohistochemistry on mice ovary. We couldn't find commercially available antibodies for these proteins suitable for IHC. Does anybody know sources for buying this items? Thanks in advance... Dr. Necat Yilmaz MD, PhD Universi

[Histonet] Thanks for capillary stain

Dear All, I'm very grateful for your kindly efforts. We've decided to stain our tissues with PAS. We couldn't perform IHC due to time limitations with the study. Because it usually takes about 2 months obtaining appropriate antibodies here. Fortunately I'm a member of this society. I wish you

[Histonet] Capillary counting

Hi All, We need a protocol demonstrating capillaries in sceletal muscle tissue other than immunohistochemistry. We're planning to compare number of capillaries between different experiment groups for evaluating angiogenesis. Is there any special stain to suggest for this purpose? Thanks in ad

[Histonet] Nikon Diaphot 300

Hello All, Could any of you provide an user or an instruction manual of a Nikon Diaphot 300 inverted fluorescence microscope preferably as a pdf file? Thanks in advance. Necat Yilmaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu ht

[Histonet] need help for cultured cells

Dear Netters, How can I set 50.000 cells in 1 ul suspension from a cultured cell pellet? I need your kindly help. Best regards... Dr. Necat Yilmaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

[Histonet] BrdU incorporating and staining

Dear Netters, We are setting up a cell therapy model in neonate rats by injecting primary cultured astrocytes intracerebrally. My questions are: 1. Is it possible to incorporate BrdU in all cells by adding it 10 uM concentration into culture media from beginning of the culture? 2. If so, can