Histonetters:
Our lab needs to paraffin embed and cut bone. Is there a special
process for fixation of bone, or can it be harvested and dropped right
into NBF?
--
Nicole Cosenza
Research Technician
Institute for Plastic Surgery
SIU School of Medicine
Springfield, Il
217.545.3862
Histonetters:
My lab is needing to lyophilize some skin tissue. Is there a company
that offers such services?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
I need to do FISH with the universal bacterial probe EUB338. I am not
too familiar with FISH. Can we fix the sample with NBF or is 4%
paraformaldehyde better? The samples are acellular dermal matrix. A
quick lit search shows people using 4% paraformaldehyde, but the
histologist that will be em
Hi all:
My lab is starting a project involving neuromas. We are in need of resin
embedding of the nerves, but our facility does not have the
capabilities. Is there a company that offers resin embedding/sectioning
services?
___
Histonet mailing list
Hello all:
I recently did IHC on paraffin embedded slides via the DAB method. Upon
addition of the DAB, the entire section turned brown. I know this is not
true staining, as I don't expect a high yield of my protein of interest.
Any suggestions as to why this happened and what to do about it?
My lab is thinking of performing a nerve study. We want to IF stain
cross sections of nerve for a few different antigens of interest. The
nerve will only be 5mm in length. Is it better to paraffin embed and
section or cryosection? Is there a way to stain the tissue to it can be
seen in the blo
We are beginning a retrograde tracing study in rats. We injected DiD
into the rats and in 4 weeks we will perfuse and harvest the brainstems.
My question is, is vibratome sectioning better than cryostat sectioning?
The literature that accompanied the dye said either is an option, but
some have
Hello all,
I am looking for opinions on how long/often I can re-use ethanols (100%,
95%, 50%) when I rehydrate paraffin sections for IHC staining.
Thanks!
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/ma
I have recently stained some muscle tissue with bromoindigo and
urea-silver to examine the neuromuscular junction. The nerve axons and
endplates stain quite well this way, but I'd like to add some color to
the slide for picture-purposes. Is there a good counterstain (eosin, or
Biebrich scarlet
I am looking into staining motor end plates. I've come across this
combined cholinesterase-silver stain (reference Pestronk and Drachman,
1978). Based on the date of the paper, I'm wondering what the current
technique is for this double staining.
Anyone currently doing AchE and axon staining
I am looking into a project involving motor end plate staining.
Literature that I've found continually references Karnovsky and Roots
from the 60s. However the papers are not supplying all the details.
Does anyone do AchE staining by this method on fresh frozen, unfixed
tissue sections? If s
We are beginning a retrograde tracing study in rats. We injected DiD
into the rats and in 4 weeks we will perfuse and harvest the brainstems.
My question is, is vibratome sectioning better than cryostat sectioning?
The literature that accompanied the dye said either is an option, but
some have
12 matches
Mail list logo