[Histonet] Fw: Reminder Anatomic Pathology Rounds today Wednesday 9/13

From: Smittle, Anne Sent: Wednesday, September 13, 2023 7:59 AM Subject: FW: Reminder Anatomic Pathology Rounds today Wednesday 9/13 Good morning, MS2 PALM Rounds today beginning around 1:00ish today in the Gross Room, continuing on into Histology Labs and finishing up in the Residents Library

[Histonet] Leica H&E 600

I would appreciate any feedback from anyone with experience using the Leica H&E 600. I recently attended a demo of the unit and we are still hung up on the large slide tray it uses instead of the traditional slide racks. I also spoke with another user who indicated that if you need to re-cover

[Histonet] Histo Chill tissue freezing bath

We were given a brand new, neve used, FTS SP Scientific Histo Chill tissue freezing bath. This will be great for our department since we won't have to deal with liquid nitrogen. Our problem is we have no idea how to use it. The company doesn't provide installation or training just an operator

[Histonet] Frozen tissue from Immunofluorescence

Would anyone be willing to share with me what you do with residual frozen tissue from immunofluorescence testing or frozen tissue remaining from muscle biopsy? CAP requirements only address what you do with residual frozen tissue remaining from intraoperative consultations. We keep the frozen

[Histonet] Problems with DIF staining of C4d

We perform manual DIF staining at our facility and C4d has always been a problem. We end up repeating the stain more times than not due to lack of staining. We aliquot the FITC and freeze at -70C until ready for use. We also started doing the same with the C4d. Sometimes it works and sometim

[Histonet] Fw: Enrichment Week Starts TODAY

Histology will get their Med Tech student tomorrow, Feb 23 at 07:00 until 11:00. From: Schmidt, Ashley A. Sent: Monday, February 22, 2021 6:57 AM Subject: Enrichment Week Starts TODAY Reminder, we have a student on Enrichment week starting today AM (7am-11)

[Histonet] HSV-2 antibody - Cell Marque was not the problem

Last week I posted an inquiry about having problems with Cell Marque's HSV-2 polyclonal antibody. I have to retract my statement that it was Cell Marque using a different antibody source. After spending two weeks troubleshooting my stainer, troubleshooting the positive control slides, yelling

[Histonet] Cell Marque HSV II

For those of you who use Cell Marques HSV II polyclonal rabbit antibody (382A-18-ASR) were you aware that they sent out information last year stating that their antibody source was discontinued and that they would be using a different antibody source (I guess the rabbit died - sorry, I just had

Re: [Histonet] Histonet Digest, Vol 169, Issue 16

We have a HistosMate from Milestone Medical. It is wonderful. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 one...@wvumedicine.org -- Message: 2 Date: Fri, 22 Dec 2017 09:19:03

[Histonet] Anti-nitrotyrosine

Would anyone have automated IHC staining protocols for Anti-nitrotyrosine? The antibody is from Santa Cruz Biotechnology #55256. Any help would be appreciated. Thanks Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC one...@wvuhealthcare.com Histology Supervisor, Technic

[Histonet] Ventana Ultra CD10

We are in the last stages of contract negotiations for purchasing two Ventana Benchmark Ultras. During this time period we have been optimizing our current inventory of approximately 110 antibodies and validating before our current instrumentation is removed within the next two weeks. My Venta

[Histonet] Ventana protocols for ISH control probes

We are optimizing/validating a new Ventana Benchmark Ultra but I need protocols for the positive RNA probe (U6 DNA Probe) and the Negative control probe. Any help would be much appreciated. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC one...@wvuhealthcare.com Histolo

[Histonet] Toluidine blue background

We would like to use Toluidine Blue for staining frozen sections of kidney biopsy. We finally are able to get the stain dark enough, but we can't get rid of the background caused by the OCT.We've washed with water, alcohols, and PBS with no success. Is anyone out there using Toluidine Blu

[Histonet] RE: Mounting media for immunofluorescence

We use Vectashield Mounting Medium, catalog# H-1000, Vector Laboratories, 10 ml. My pathologists absolutely love it. It really works for retarding photobleaching and really preserves fluorescence. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC one...@wvuhealthcare.com

[Histonet] Slide Mate Print Quality

In answer to the question about the Slide Mate printing coming off of the slides, we had the same issue about a year ago. At first Thermo though it was the ribbon but it turned out the problem was with the slides. We use the Shandon Superfrost Plus (Cat# 6776214). We contacted Thermo/Shandon

[Histonet] RE: ANP.22970

Per CAP checklist ANP.22970, it lists the expected published benchmarks for ER/PR, does anyone know where to find them for Her2 or MMR? Thanks and Happy Friday!! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph

[Histonet] CAP Annual Results Comparison for FISH/ISH

Would fellow Histonetters be able to explain how they answer the following CAP question: ANP.22970 For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates in