[Histonet] hematoxylin stain for polyester wax sections

2010-09-22 Thread Onstead-Cardinale, Luisa
Can anyone suggest a good hematoxylin counterstain for central nervous system tissue embedded in polyester wax? And provide a recipe? Thank you Luisa Luisa Onstead-Cardinale, M.S. Research Associate UF Biomedical Research Laboratory, Room 121 655 West 11th Street Jacksonville, Fl 32206

[Histonet] Steedman polyester wax

2010-09-20 Thread Onstead-Cardinale, Luisa
I would like to know if anyone has a good protocol that includes tissue fixation (preferred fixative, fixation temperature etc) and tissue processing (time etc.) for low melting Steedman polyester wax . The tissue to be embedded is chick embryo brain. I am going to do all the tissue processin